PDF(Pubmed)
Abstract：
UNASSIGNED: Muscle atrophy due to immobility is a common complication of many diseases and a consequence of therapeutic processes. Immobility and inactivity have been shown to be associated with increased inflammation. The aim of this study was to investigate the therapeutic potential of Wild Bitter Melon (WBM) (Momordica charantia Linn) on muscle atrophy due to immobility in a mouse model.
UNASSIGNED: This study was performed in two phases of atrophy and recovery on male BALB/c mice which were divided into 3 groups: control, immobilized, and experimental. The treatment period with WBM at a dose of 400 mg/kg daily by gavage was 17 days, including 7 days of being immobilized and 10 days of recovery. At the end of each phase, half of the mice from each group were examined regarding the four limb grip strength, and then histological and biochemical analyses were done.
UNASSIGNED: The tissue level of malondialdehyde (MDA) oxidative stress index in the atrophy phase in the atrophy group (5.4567±0.522) nmol/g compared to the control group (3.455±0.065) nmol significantly (p 0.001) <) increased. Also, the tissue level of MDA in the WBM group (3.87±0.035) showed a significant decrease compared to the atrophy group (p<0.01). The strength percentage of four limbs in the mice of the treatment group (-23.46±2.45) was significantly higher than that of the atrophy group (-30.60±3.15) at the end of the atrophy phase.
UNASSIGNED: The results suggest that the use of WBM reduces the degree of inflammation, oxidative stress and muscle damage, as well as muscle atrophy, which may improve the muscle atrophy in mice.
UNASSIGNED: This study was performed in two phases of atrophy and recovery on male BALB/c mice which were divided into 3 groups: control, immobilized, and experimental. The treatment period with WBM at a dose of 400 mg/kg daily by gavage was 17 days, including 7 days of being immobilized and 10 days of recovery. At the end of each phase, half of the mice from each group were examined regarding the four limb grip strength, and then histological and biochemical analyses were done.
UNASSIGNED: The tissue level of malondialdehyde (MDA) oxidative stress index in the atrophy phase in the atrophy group (5.4567±0.522) nmol/g compared to the control group (3.455±0.065) nmol significantly (p 0.001) <) increased. Also, the tissue level of MDA in the WBM group (3.87±0.035) showed a significant decrease compared to the atrophy group (p<0.01). The strength percentage of four limbs in the mice of the treatment group (-23.46±2.45) was significantly higher than that of the atrophy group (-30.60±3.15) at the end of the atrophy phase.
UNASSIGNED: The results suggest that the use of WBM reduces the degree of inflammation, oxidative stress and muscle damage, as well as muscle atrophy, which may improve the muscle atrophy in mice.
摘要：
■由于不动而引起的肌肉萎缩是许多疾病的常见并发症，也是治疗过程的结果。已显示不活动和不活动与炎症增加有关。这项研究的目的是研究野生苦瓜（WBM）（苦瓜）对小鼠模型中由于不动而引起的肌肉萎缩的治疗潜力。
■这项研究是在雄性BALB/c小鼠的萎缩和恢复两个阶段进行的，分为3组：对照组，固定不动,和实验。WBM每天400mg/kg剂量的灌胃治疗期为17天，包括7天的固定和10天的恢复。在每个阶段结束时,每组一半的小鼠进行了关于四个肢体握力的检查，然后进行组织学和生化分析。
■萎缩组萎缩期组织丙二醛(MDA)氧化应激指数水平(5.4567±0.522)nmol/g较对照组(3.455±0.065)nmol显著升高(p<0.001)。此外，与萎缩组相比，WBM组的组织MDA水平（3.87±0.035）显着降低（p<0.01）。萎缩期结束时,治疗组小鼠四肢力量百分比(-23.46±2.45)显著高于萎缩组(-30.60±3.15)。
■结果表明，使用WBM可以降低炎症程度，氧化应激和肌肉损伤，以及肌肉萎缩，这可能会改善小鼠的肌肉萎缩。
■这项研究是在雄性BALB/c小鼠的萎缩和恢复两个阶段进行的，分为3组：对照组，固定不动,和实验。WBM每天400mg/kg剂量的灌胃治疗期为17天，包括7天的固定和10天的恢复。在每个阶段结束时,每组一半的小鼠进行了关于四个肢体握力的检查，然后进行组织学和生化分析。
■萎缩组萎缩期组织丙二醛(MDA)氧化应激指数水平(5.4567±0.522)nmol/g较对照组(3.455±0.065)nmol显著升高(p<0.001)。此外，与萎缩组相比，WBM组的组织MDA水平（3.87±0.035）显着降低（p<0.01）。萎缩期结束时,治疗组小鼠四肢力量百分比(-23.46±2.45)显著高于萎缩组(-30.60±3.15)。
■结果表明，使用WBM可以降低炎症程度，氧化应激和肌肉损伤，以及肌肉萎缩，这可能会改善小鼠的肌肉萎缩。
