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Abstract：
UNASSIGNED: Staphylococcus aureus is an important pathogen that causes mild to invasive infections in hospitals and the community. Although methicillin-susceptible Staphylococcus aureus (MSSA) isolates continue to cause different infections, there is no data on the genetic backgrounds of the MSSA colonizing or causing infections in Kuwait hospitals. This study aimed to investigate MSSA isolated from patients admitted to Kuwait hospitals for antibiotic resistance and genetic backgrounds to understand their clonal composition.
UNASSIGNED: Consecutive MSSA isolates were collected from single patients during two surveillance periods in 2016 and 2021 in 13 public hospitals. The isolates were characterized using antibiogram, staphylococcal protein A (spa) typing, DNA microarray analysis, and multilocus sequence typing (MLST) using standard protocols.
UNASSIGNED: A total of 446 MSSA was cultured from different clinical samples in 2016 (n = 240) and 2021 (n = 206). All isolates were susceptible to vancomycin [minimum inhibitory concentration (MIC) ≤ 2 mg/L], teicoplanin (MIC ≤2 mg/L), linezolid (MIC ≤4 mg/L), ceftaroline (MIC ≤2 mg/L), rifampicin, and mupirocin but were resistant to erythromycin (21.3%), clindamycin (14.0%), gentamicin (3.8%), kanamycin (10.5%), fusidic acid (27.0%), tetracycline (6.9%), trimethoprim (23.1%), and ciprofloxacin (35.2%). Molecular typing identified 155 spa types, dominated by t127 (15.0%), t084 (5.4%), t3841 (5.4%), t267 (2.4%), t442 (2.2%), t091 (2.2%), t021 (2.2%), and t003 (2.2%); 31 clonal complexes (CCs); and 56 sequence types (STs). The majority of the isolates (n = 265; 59.4%) belonged to CC1 (20.6%), CC15 (10.9%), CC22 (5.1%), CC30 (7.6%), CC361 (10.1%), and CC398 (4.7%).
UNASSIGNED: The MSSA isolates belonged to diverse genetic backgrounds dominated by CC1, CC15, CC22, CC30, CC361, and CC398. The distribution of MSSA clones in 2016 and 2021 showed the stability of these clones over time. The study provides the first comprehensive data on the clonal distribution of MSSA in Kuwait hospitals.
UNASSIGNED: Consecutive MSSA isolates were collected from single patients during two surveillance periods in 2016 and 2021 in 13 public hospitals. The isolates were characterized using antibiogram, staphylococcal protein A (spa) typing, DNA microarray analysis, and multilocus sequence typing (MLST) using standard protocols.
UNASSIGNED: A total of 446 MSSA was cultured from different clinical samples in 2016 (n = 240) and 2021 (n = 206). All isolates were susceptible to vancomycin [minimum inhibitory concentration (MIC) ≤ 2 mg/L], teicoplanin (MIC ≤2 mg/L), linezolid (MIC ≤4 mg/L), ceftaroline (MIC ≤2 mg/L), rifampicin, and mupirocin but were resistant to erythromycin (21.3%), clindamycin (14.0%), gentamicin (3.8%), kanamycin (10.5%), fusidic acid (27.0%), tetracycline (6.9%), trimethoprim (23.1%), and ciprofloxacin (35.2%). Molecular typing identified 155 spa types, dominated by t127 (15.0%), t084 (5.4%), t3841 (5.4%), t267 (2.4%), t442 (2.2%), t091 (2.2%), t021 (2.2%), and t003 (2.2%); 31 clonal complexes (CCs); and 56 sequence types (STs). The majority of the isolates (n = 265; 59.4%) belonged to CC1 (20.6%), CC15 (10.9%), CC22 (5.1%), CC30 (7.6%), CC361 (10.1%), and CC398 (4.7%).
UNASSIGNED: The MSSA isolates belonged to diverse genetic backgrounds dominated by CC1, CC15, CC22, CC30, CC361, and CC398. The distribution of MSSA clones in 2016 and 2021 showed the stability of these clones over time. The study provides the first comprehensive data on the clonal distribution of MSSA in Kuwait hospitals.
摘要：
■金黄色葡萄球菌是一种重要的病原体，可在医院和社区中引起轻度至侵袭性感染。尽管甲氧西林敏感的金黄色葡萄球菌（MSSA）分离株继续引起不同的感染，没有关于科威特医院中MSSA定殖或引起感染的遗传背景的数据。这项研究旨在调查从科威特医院收治的患者中分离出的MSSA的抗生素耐药性和遗传背景，以了解其克隆组成。
■在2016年和2021年的两个监测期间，在13家公立医院中从单个患者中收集了连续的MSSA分离株。分离株的特征是使用抗菌谱，葡萄球菌蛋白A（spa）分型，DNA微阵列分析,和使用标准方案的多位点序列分型(MLST)。
■在2016年（n=240）和2021年（n=206）从不同的临床样本中总共培养了446MSSA。所有菌株均对万古霉素敏感[最低抑菌浓度(MIC)≤2mg/L],替考拉宁（MIC≤2mg/L），利奈唑胺（MIC≤4mg/L），头孢洛林（MIC≤2mg/L），利福平,和莫匹罗星，但对红霉素耐药(21.3%)，克林霉素（14.0%），庆大霉素(3.8%),卡那霉素(10.5%)，夫西地酸（27.0%），四环素(6.9%),甲氧苄啶（23.1％），环丙沙星(35.2%)。分子分型确定了155种温泉类型，以T127（15.0%）为主，t084（5.4%），t3841（5.4%），t267（2.4%），t442（2.2%），t091（2.2%），t021（2.2%），和t003（2.2％）；31个克隆复合物（CC）；和56个序列类型（STs）。大多数分离株（n=265；59.4％）属于CC1（20.6％），CC15（10.9%），CC22（5.1%），CC30（7.6%），CC361（10.1%），和CC398（4.7%）。
■MSSA分离株属于以CC1、CC15、CC22、CC30、CC361和CC398为主的不同遗传背景。2016年和2021年MSSA克隆的分布显示了这些克隆随时间的稳定性。该研究提供了有关科威特医院MSSA克隆分布的第一个全面数据。
■在2016年和2021年的两个监测期间，在13家公立医院中从单个患者中收集了连续的MSSA分离株。分离株的特征是使用抗菌谱，葡萄球菌蛋白A（spa）分型，DNA微阵列分析,和使用标准方案的多位点序列分型(MLST)。
■在2016年（n=240）和2021年（n=206）从不同的临床样本中总共培养了446MSSA。所有菌株均对万古霉素敏感[最低抑菌浓度(MIC)≤2mg/L],替考拉宁（MIC≤2mg/L），利奈唑胺（MIC≤4mg/L），头孢洛林（MIC≤2mg/L），利福平,和莫匹罗星，但对红霉素耐药(21.3%)，克林霉素（14.0%），庆大霉素(3.8%),卡那霉素(10.5%)，夫西地酸（27.0%），四环素(6.9%),甲氧苄啶（23.1％），环丙沙星(35.2%)。分子分型确定了155种温泉类型，以T127（15.0%）为主，t084（5.4%），t3841（5.4%），t267（2.4%），t442（2.2%），t091（2.2%），t021（2.2%），和t003（2.2％）；31个克隆复合物（CC）；和56个序列类型（STs）。大多数分离株（n=265；59.4％）属于CC1（20.6％），CC15（10.9%），CC22（5.1%），CC30（7.6%），CC361（10.1%），和CC398（4.7%）。
■MSSA分离株属于以CC1、CC15、CC22、CC30、CC361和CC398为主的不同遗传背景。2016年和2021年MSSA克隆的分布显示了这些克隆随时间的稳定性。该研究提供了有关科威特医院MSSA克隆分布的第一个全面数据。
