UNASSIGNED: In this study, we designed second-generation CAR T cells targeting FAP with 4-1BB as a co-stimulatory molecule, and tested their cytotoxicity against FAP-positive cells (hFAP-HT1080 cells and a variety of primary CAFs) in vitro and in Cell line-derived xenograft (CDX) and a patient-derived xenograft (PDX) model.
UNASSIGNED: Results showed that our FAP-CAR T cells were powerfully potent in killing human and murine FAP-positive tumor cells and CAFs in multiple types of tumors in BALB/c and C57BL/6 mice and in patient-derived xenografts (PDX) model. And they were proved to be biologically safe and exhibit low-level OTOT.
UNASSIGNED: Taken together, the human/murine cross-reactive FAP-CAR T cells were powerfully potent in killing human and murine FAP positive tumor cells and CAFs. They were biologically safe and exhibit low-level OTOT, warranting further clinical investigation into our FAP-CAR T cells.
■在这项研究中,我们设计了以4-1BB作为共刺激分子靶向FAP的第二代CAR-T细胞,并在体外和细胞系来源的异种移植物(CDX)和患者来源的异种移植物(PDX)模型中测试了它们对FAP阳性细胞(hFAP-HT1080细胞和各种原代CAF)的细胞毒性。
■结果表明,我们的FAP-CART细胞在BALB/c和C57BL/6小鼠和患者衍生的异种移植物(PDX)模型中的多种肿瘤中具有强大的杀伤人和鼠FAP阳性肿瘤细胞和CAF的能力。它们被证明是生物安全的,并且表现出低水平的OTOT。
■放在一起,人/鼠交叉反应性FAP-CART细胞在杀伤人和鼠FAP阳性肿瘤细胞和CAFs方面非常有效.它们是生物安全的,表现出低水平的OTOT,保证对我们的FAP-CART细胞进行进一步的临床研究。