Abstract：
Food allergy and eczema are the earliest allergic phenotypes in childhood. These diseases could be related to either IgE-mediated or non-IgE-mediated reactions to the allergen. TNFRSF17 is a key molecule in B cell maturation and is important in both types of responses.We conducted a study comparing the relative expression and the methylation status at the TNFRSF17 in regard to the child\'s early atopic sensitisation and allergic phenotypes.In the recruited population of 200 women and 174 children with available clinical data (physical examination by allergist and antigen-specific IgE measurements), 78 cord blood samples were included in the gene expression analysis (relative gene expression with GAPDH as reference by RT-PCR) and 96 samples with microarray DNA methylation data (whole genome methylation profile Infinium MethylationEPIC).The altered TNFRSF17 methylation pattern in the cord blood at both single cg04453550 and mean methylation at upstream of TNFRSF17 was observed in children who developed food allergy and/or eczema in early childhood. The change in methylation profile was mirrored by the relative expression. The profile of IgE sensitisation to food and/or inhalant allergens was not significantly associated with either methylation or expression of TNFRSF17.In conclusion, methylation at the upstream sites at TNFRSF17 in the cord blood at birth is associated with food allergy and eczema early in childhood.
摘要：
食物过敏和湿疹是儿童期最早的过敏表型。这些疾病可能与IgE介导的或非IgE介导的对变应原的反应有关。TNFRSF17是B细胞成熟的关键分子，在两种类型的反应中都很重要。我们进行了一项研究，比较了儿童早期特应性致敏和过敏性表型的TNFRSF17的相对表达和甲基化状态。在招募的200名妇女和174名儿童中，有可用的临床数据（通过变态反应者和抗原特异性IgE测量进行的体格检查），78个脐带血样品包括在基因表达分析中(通过RT-PCR以GAPDH作为参考的相对基因表达)和96个样品包括微阵列DNA甲基化数据(全基因组甲基化谱Infinium甲基化EPIC)。在儿童早期发生食物过敏和/或湿疹的儿童中，观察到脐带血中单个cg04453550和TNFRSF17上游平均甲基化模式的改变。相对表达反映了甲基化谱的变化。IgE对食物和/或吸入性过敏原的致敏特性与TNFRSF17的甲基化或表达无显著关联。总之,出生时脐带血TNFRSF17上游位点的甲基化与儿童早期食物过敏和湿疹相关.
