Abstract：
Sodium tanshinone IIA sulfonate (STS), which is extracted from a Chinese medicinal herb, possesses many pharmacologic functions, such as coronary dilation, anti-inflammatory properties, and antiapoptotic and antioxidant effects. It remains unknown whether STS can protect cardiomyocytes injured after radiation therapy. An in vitro Sprague-Dawley (SD) rat neonatal cardiomyocyte system was established. Primary cardiomyocytes (PCMs) from neonatal SD rats were isolated under sterile conditions. PCM cells were divided into a control group (0 Gy/hour) and 5 experimental radiation therapy groups (0.25 Gy/hour, 0.5 Gy/hour, 1 Gy/hour, 2 Gy/hour, and 4 Gy/hour). Cell viability, the content of malondialdehyde (MDA), the lactate dehydrogenase (LDH) leakage rate, and superoxide dismutase (SOD) and glutathione (GSH) activities were recorded separately in each group after 7 days of culture. Western blot was used to detect the levels of p38, caspase-3 protein, and X protein (BAX) associated with B-cell lymphoma 2 (Bcl-2) in PCMs. X-rays inhibited cell growth, decreased cell viability, and induced an oxidative stress response in PCMs. STS and SB203580 (the inhibitor of P38 mitogen-activated protein kinase pathway) alleviated X-ray-induced damage to PCMs. An enzyme-linked immunosorbent assay showed that X-rays increased the cTnT level. STS and SB203580 ameliorated the X-ray-induced increase in cTnT leakage. X-rays enhanced the expression of p38/p-p38 and caspase-3 while reducing the expression of Bcl-2/BAX in PCMs, as demonstrated by western blotting. STS and SB203580 mitigated the changes in protein expression triggered by X-ray radiation. In conclusions, STS was shown to exert significant cardioprotective, anti-inflammatory, and antioxidant effects in PCMs by inhibiting the p38 mitogen-activated protein kinase pathway.
摘要：
丹参酮IIA磺酸钠（STS），它是从中草药中提取的，具有许多药理功能，如冠状动脉扩张，抗炎特性，以及抗凋亡和抗氧化作用。尚不清楚STS是否可以保护放射治疗后受伤的心肌细胞。建立了体外Sprague-Dawley（SD）大鼠新生心肌细胞系统。在无菌条件下分离来自新生SD大鼠的原代心肌细胞(PCM)。将PCM细胞分为对照组（0Gy/h）和5个实验性放射治疗组（0.25Gy/h，0.5Gy/小时,1Gy/小时,2Gy/小时,和4Gy/小时)。细胞活力，丙二醛（MDA）的含量，乳酸脱氢酶（LDH）泄漏率，培养7天后分别记录各组超氧化物歧化酶（SOD）和谷胱甘肽（GSH）活性。Westernblot检测p38、caspase-3蛋白水平,和X蛋白（BAX）与PCMs中的B细胞淋巴瘤2（Bcl-2）相关。X射线抑制细胞生长，细胞活力下降，并在PCM中引起氧化应激反应。STS和SB203580（P38丝裂原活化蛋白激酶途径的抑制剂）减轻了X射线对PCM的损伤。酶联免疫吸附试验显示X射线增加了cTnT水平。STS和SB203580改善了X射线引起的cTnT泄漏增加。X射线增强PCMs中p38/p-p38和caspase-3的表达，同时降低Bcl-2/BAX的表达，正如西方印迹所证明的。STS和SB203580减轻了由X射线辐射引发的蛋白质表达的变化。在结论中，STS被证明具有显著的心脏保护作用，抗炎,通过抑制p38丝裂原活化蛋白激酶途径在PCM中的抗氧化作用。
