Abstract：
Spodoptera frugiperda is a notorious invasive pest causing substantial yield losses of crops and has developed resistance to various types of insecticides. In this study, a cyantraniliprole-resistant strain, SfCYAN-R, was obtained from a susceptible strain, SfCYAN-S, after 13 generations of selection with cyantraniliprole. The fitness cost in SfCYAN-R strain was evaluated, and the putative resistance-related genes were explored by RNA-seq analysis. The results showed that SfCYAN-R strain developed 23.97-fold resistance to cyantraniliprole with the realistic heritability of 0.127. The development time of eggs, larvae, prepupae and pupae in SfCYAN-R strain was significantly prolonged than that in SfCYAN-S strain, but no difference in pupation rate, emergence rate and female fecundity was observed between SfCYAN-R and SfCYAN-S strains. Comparative gene expression analysis between SfCYAN-R and SfCYAN-S strains identified 776 significant differentially expressed genes (DEGs), among which several DEGs associated with xenobiotic metabolism were upregulated in SfCYAN-R strain. These results provide insights into the resistance mechanisms of cyantraniliprole and would be helpful for resistance management of S. frugiperda.
摘要：
节食夜蛾是一种臭名昭著的侵袭性害虫，会导致农作物的大量产量损失，并且对各种类型的杀虫剂产生了抗性。在这项研究中,一种抗氰药的菌株，SfCYAN-R,是从易感菌株获得的，Sfcyan-S,经过13代的选择与cyantraniliprole。评估了SfCYAN-R菌株的适应性成本，并通过RNA-seq分析探索推定的抗性相关基因。结果表明，SfCYAN-R菌株对蓝藻的抗性为23.97倍，实际遗传力为0.127。卵的发育时间,幼虫,SfCYAN-R菌株的预p和p明显长于SfCYAN-S菌株，但是化蛹率没有差异,在SfCYAN-R和SfCYAN-S菌株之间观察到出苗率和雌性繁殖力。SfCYAN-R和SfCYAN-S菌株之间的比较基因表达分析确定了776个显着差异表达基因（DEGs），其中SfCYAN-R菌株中几个与异源生物代谢相关的DEGs上调。这些结果为蓝藻的抗性机制提供了见解，并将有助于S.frugiperda的抗性管理。
