Abstract：
Acephate and chlorantraniliprole are two insecticides widely used in agricultural applications. Several studies were focused on the mode of action and related biological and cellular level expressions. However, the sub-lethal dose and related molecular expression level of acephate and chlorantraniliprole have not been evaluated or studied to the same degree. In this study, we investigated the sub-lethal toxicity of acephate and chlorantraniliprole in Drosophila melanogaster. The EC50 value was recorded with high difference, and is found to be 1.9 μg/ml and 0.029 μg/ml respectively for acephate and chlorantraniliprole, the difference is simply because of the different modes of action. The 1/5th EC50 concentration was selected for studying the pesticide induced transcriptomics in D. melanogaster. Both pesticides significantly altered the expression profile of several transcripts which are involved in proteolysis, detoxification, chromosome associated proteins and immune response genes and so on. The effect of both pesticides on D. melanogaster was further explored by screening the genes involved in toxicity, which were analyzed using, GO and KEGG pathways. The results revealed that the sub-lethal exposure of both pesticides caused significant changes in the global gene transcription profiles and each pesticide had their unique mode of alteration in the D. melanogaster.
摘要：
乙酰甲胺磷和氯丁虫是两种广泛用于农业应用的杀虫剂。一些研究集中在作用方式以及相关的生物和细胞水平表达上。然而,乙酰甲胺磷和氯硝唑的亚致死剂量和相关分子表达水平尚未得到相同程度的评估或研究。在这项研究中,我们研究了乙酰甲胺磷和氯丁虫对果蝇的亚致死毒性。EC50值记录为高差异，并且发现乙酰甲胺磷和氯蒽环丙酯分别为1.9μg/ml和0.029μg/ml，这种差异仅仅是因为行动模式的不同。选择1/5EC50浓度用于研究黑腹D中农药诱导的转录组学。两种农药都显着改变了参与蛋白水解的几种转录物的表达谱，排毒,染色体相关蛋白和免疫应答基因等。通过筛选与毒性有关的基因，进一步探索了两种农药对黑腹的影响。使用分析，GO和KEGG途径。结果表明，两种农药的亚致死暴露导致全球基因转录谱发生显着变化，每种农药在黑腹D中都有其独特的改变方式。
