Abstract：
It has been demonstrated that ferulic acid (FA) can be effectively encapsulated using wheat gluten amyloid fibrils (AF) and chitosan (CS) in a double network hydrogel (DN) form, with cross-linking mediated by Genipin (GP). Within this system, the DN comprising gluten AF-FA and CS-FA exhibited optimal loading metrics at a formulation designated as DN8, achieving a load efficiency of 88.5 % and a load capacity of 0.78 %. Analysis through fluorescence quenching confirmed that DN8 harbored the highest quantity of FA. Fourier-transform infrared spectroscopy (FTIR) further verified a significant increase in β-sheet content post-hydrogel formation, enhancing the binding capacity for FA. Rheological assessments indicated a transition from solution to gel, delineating the phase state of the DN. Comprehensive in vitro digestion studies revealed that DN8 provided superior sustained release properties, exhibited the highest total antioxidant capacity, and displayed potent inhibitory activities against angiotensin I converting enzyme (ACE) and acetylcholinesterase (Ach-E). Additionally, the DN significantly bolstered the stability of FA against photothermal degradation. Collectively, these findings lay foundational insights for the advancement of the wheat gluten AF-based delivery system for bioactive compounds and provided a theoretical basis for the development of functional foods.
摘要：
已经证明，使用双网络水凝胶（DN）形式的小麦面筋淀粉样纤维（AF）和壳聚糖（CS）可以有效地包封阿魏酸（FA），与京尼平（GP）介导的交联。在这个系统中,包含谷蛋白AF-FA和CS-FA的DN在指定为DN8的制剂下表现出最佳的负载度量,实现88.5%的负载效率和0.78%的负载能力。通过荧光猝灭分析证实DN8含有最高量的FA。傅里叶变换红外光谱（FTIR）进一步证实了水凝胶形成后β-折叠含量的显着增加，增强FA的约束力。流变学评估表明从溶液到凝胶的转变，描述DN的相位状态。全面的体外消化研究表明，DN8具有优异的缓释性能，表现出最高的总抗氧化能力，并显示出对血管紧张素I转换酶（ACE）和乙酰胆碱酯酶（Ach-E）的有效抑制活性。此外,DN显着增强了FA对光热降解的稳定性。总的来说，这些发现为基于小麦面筋蛋白AF的生物活性化合物传递系统的发展奠定了基础，并为功能食品的开发提供了理论依据。
