Abstract：
Unlike natural soyasaponins and their aglycones formed by enzymatic hydrolysis in the human intestine, in vivo intermediate soyasaponin metabolites are difficult to prepare. Therefore, the pharmacological activities of in vivo intermediate soyasaponins remain uninvestigated. Herein, in vivo intermediate soyasaponins with purities of >90% were prepared by in vitro deacetylation (alkaline treatment) and deglucosylation (β-glucosidase treatment) of natural soyasaponins using preparative high-performance liquid chromatography. These compounds exhibited higher anti-inflammatory and antioxidant activities than natural soyasaponins in in vitro bioassays, suggesting that the intermediate soyasaponins can be used as improved bioactive food supplements. To the best of our knowledge, this is the first study reporting the in vitro preparation and bioassays of in vivo intermediate soyasaponin metabolites formed in the human intestine.
摘要：
与天然大豆皂甙及其在人体肠道中通过酶促水解形成的苷元不同，体内中间大豆皂甙代谢物很难制备。因此,体内中间大豆皂甙的药理活性仍未研究。在这里，通过制备型高效液相色谱法对天然大豆皂甙进行体外脱乙酰（碱处理）和脱葡糖基化（β-葡萄糖苷酶处理），制备纯度>90％的体内中间大豆皂甙。这些化合物在体外生物测定中表现出比天然大豆皂甙更高的抗炎和抗氧化活性，这表明中间大豆皂甙可以用作改进的生物活性食品补充剂。据我们所知,这是首次报道人体肠道中形成的体内大豆皂甙代谢产物的体外制备和生物分析。
