PDF(Pubmed)
Abstract：
UNASSIGNED: To investigate the effects of anterior chamber pigment dispersion on ocular immune privilege and the possible mechanisms involved in a DBA/2J mouse model of pigmentary glaucoma.
UNASSIGNED: DBA/2J mice were utilized as a pigment dispersion model, and age-matched C57BL/6J mice were used as the control group in this study. Proteins in the aqueous humor (AH) and serum were quantified using the bicinchoninic acid assay. Immune cells in the AH were detected using hematoxylin and eosin staining and immunocytochemistry. The expression of TGF-β2 in the AH and cytokine levels (IL-10, IFN-γ) in serum were measured using ELISA. Anterior chamber-associated immune deviation (ACAID) was induced in DBA/2J mice by injecting antigens into the anterior chamber. Delayed-type hypersensitivity (DTH) assays were used to assess the induction of ACAID. In DBA/2J mice, before and after pigment dispersion, following anterior chamber injection of pigment particles, and after ACAID modeling, the expression of regulatory T cells (Tregs) was detected using flow cytometry.
UNASSIGNED: Compared to C57BL/6J mice, the protein concentration, immune cell count, and TGF-β2 levels in the AH were elevated in DBA/2J mice. Protein concentration and IL-10 levels in serum were increased, while IFN-γ levels were decreased in DBA/2J. Additionally, the expression of Treg cells in the spleen of DBA/2J mice was significantly increased after pigment dispersion and anterior chamber injection of pigment particles. At 3 and 6 months, DTH responses in DBA/2J mice were not inhibited, thus preventing ACAID induction. However, the opposite was observed at 9 months in DBA/2J mice. Furthermore, the ACAID group exhibited an augmented expression of Treg cells.
UNASSIGNED: Dispersion of pigment particles in the anterior chamber of the eye enhances the state of ocular immune privilege by influencing the immunosuppressive microenvironment and inducing more Treg cells to reestablish ACAID.
UNASSIGNED: DBA/2J mice were utilized as a pigment dispersion model, and age-matched C57BL/6J mice were used as the control group in this study. Proteins in the aqueous humor (AH) and serum were quantified using the bicinchoninic acid assay. Immune cells in the AH were detected using hematoxylin and eosin staining and immunocytochemistry. The expression of TGF-β2 in the AH and cytokine levels (IL-10, IFN-γ) in serum were measured using ELISA. Anterior chamber-associated immune deviation (ACAID) was induced in DBA/2J mice by injecting antigens into the anterior chamber. Delayed-type hypersensitivity (DTH) assays were used to assess the induction of ACAID. In DBA/2J mice, before and after pigment dispersion, following anterior chamber injection of pigment particles, and after ACAID modeling, the expression of regulatory T cells (Tregs) was detected using flow cytometry.
UNASSIGNED: Compared to C57BL/6J mice, the protein concentration, immune cell count, and TGF-β2 levels in the AH were elevated in DBA/2J mice. Protein concentration and IL-10 levels in serum were increased, while IFN-γ levels were decreased in DBA/2J. Additionally, the expression of Treg cells in the spleen of DBA/2J mice was significantly increased after pigment dispersion and anterior chamber injection of pigment particles. At 3 and 6 months, DTH responses in DBA/2J mice were not inhibited, thus preventing ACAID induction. However, the opposite was observed at 9 months in DBA/2J mice. Furthermore, the ACAID group exhibited an augmented expression of Treg cells.
UNASSIGNED: Dispersion of pigment particles in the anterior chamber of the eye enhances the state of ocular immune privilege by influencing the immunosuppressive microenvironment and inducing more Treg cells to reestablish ACAID.
摘要：
■研究前房色素分散对DBA/2J色素性青光眼小鼠模型眼部免疫特权的影响及其可能的机制。
■DBA/2J小鼠用作色素分散模型，以年龄匹配的C57BL/6J小鼠作为对照组。使用二辛可宁酸测定法定量房水（AH）和血清中的蛋白质。使用苏木精和伊红染色和免疫细胞化学检测AH中的免疫细胞。ELISA检测AH中TGF-β2的表达和血清中细胞因子（IL-10，IFN-γ）的水平。通过将抗原注射到前房中，在DBA/2J小鼠中诱导前房相关免疫偏差（ACAID）。延迟型超敏反应(DTH)测定用于评估ACAID的诱导。在DBA/2J小鼠中，颜料分散之前和之后，前房注射色素颗粒后，在ACAID建模之后，使用流式细胞术检测调节性T细胞(Tregs)的表达。
■与C57BL/6J小鼠相比，蛋白质浓度,免疫细胞计数，DBA/2J小鼠AH中TGF-β2水平升高。血清中蛋白质浓度和IL-10水平升高,而IFN-γ水平在DBA/2J中降低。此外,色素分散和前房注射色素颗粒后，DBA/2J小鼠脾脏中Treg细胞的表达明显增加。在3个月和6个月时，DBA/2J小鼠的DTH反应没有被抑制，从而防止丙烯酸诱导。然而,在DBA/2J小鼠中9个月时观察到相反的情况。此外，ACAID组表现出Treg细胞表达增强。
■色素颗粒在眼前房中的分散通过影响免疫抑制微环境并诱导更多的Treg细胞重建ACAID来增强眼部免疫特权状态。
■DBA/2J小鼠用作色素分散模型，以年龄匹配的C57BL/6J小鼠作为对照组。使用二辛可宁酸测定法定量房水（AH）和血清中的蛋白质。使用苏木精和伊红染色和免疫细胞化学检测AH中的免疫细胞。ELISA检测AH中TGF-β2的表达和血清中细胞因子（IL-10，IFN-γ）的水平。通过将抗原注射到前房中，在DBA/2J小鼠中诱导前房相关免疫偏差（ACAID）。延迟型超敏反应(DTH)测定用于评估ACAID的诱导。在DBA/2J小鼠中，颜料分散之前和之后，前房注射色素颗粒后，在ACAID建模之后，使用流式细胞术检测调节性T细胞(Tregs)的表达。
■与C57BL/6J小鼠相比，蛋白质浓度,免疫细胞计数，DBA/2J小鼠AH中TGF-β2水平升高。血清中蛋白质浓度和IL-10水平升高,而IFN-γ水平在DBA/2J中降低。此外,色素分散和前房注射色素颗粒后，DBA/2J小鼠脾脏中Treg细胞的表达明显增加。在3个月和6个月时，DBA/2J小鼠的DTH反应没有被抑制，从而防止丙烯酸诱导。然而,在DBA/2J小鼠中9个月时观察到相反的情况。此外，ACAID组表现出Treg细胞表达增强。
■色素颗粒在眼前房中的分散通过影响免疫抑制微环境并诱导更多的Treg细胞重建ACAID来增强眼部免疫特权状态。
