RESULTS: In this study, we have investigated the expression of the TET3 gene along with IDH1/2 genes involved in the Krebs cycle in the peripheral blood of 32 H&NC patients compared to 32 healthy controls. We estimated serum levels of TET3 protein and vitamin C and 5-hydroxymethylcytosine (5-hmC) percentage in DNA isolated from EDTA blood samples. Our findings revealed that TET3 and IDH1/2 were downregulated in H&NC patients compared to healthy controls. Serum levels of TET3 and Vitamin C were low in H&NC patients compared to healthy controls. Diminished levels of percentage 5-hmC were detected in EDTA blood samples of H&NC patients compared to controls. Spearman correlation analysis revealed a significant positive correlation between TET3 levels, vitamin C levels and 5-hmC percentage.
CONCLUSIONS: The low levels of Vitamin C are believed to contribute to decreased activity of the TET3 gene and less conversion of 5-methylcytosine (5-mC) to 5-hmC. Dietary supplementation of Vitamin C may increase TET3 activity.
结果:在这项研究中,我们调查了32例H&NC患者外周血中TET3基因和与Krebs周期相关的IDH1/2基因的表达,并与32例健康对照进行比较.我们估计了从EDTA血液样品中分离的DNA中TET3蛋白和维生素C以及5-羟甲基胞嘧啶(5-hmC)百分比的血清水平。我们的发现表明,与健康对照组相比,H&NC患者的TET3和IDH1/2下调。与健康对照相比,H&NC患者的血清TET3和维生素C水平较低。与对照相比,在H&NC患者的EDTA血液样品中检测到降低的5-hmC百分比水平。Spearman相关分析显示TET3水平之间存在显著正相关,维生素C水平和5-hmC百分比。
结论:低水平的维生素C被认为有助于降低TET3基因的活性和较少的5-甲基胞嘧啶(5-mC)转化为5-hmC。饮食补充维生素C可能会增加TET3活性。