Abstract：
BACKGROUND: Regenerative endodontics involves the use of various root canal medicaments and scaffolds, which may cause crown discoloration.
OBJECTIVE: This study aimed to investigate the combined crown discoloration of scaffolds [platelet-rich fibrin (PRF) and blood clot] applied after administration of different medicaments [modified triple antibiotic paste including doxycycline (mTAPd), modified double antibiotic paste (mDAP), calcium hydroxide (CH), and propolis].
METHODS: In total, 100 human mandibular premolar teeth were selected and prepared. The teeth were apically resected to simulate immature teeth. The positive and negative control groups (n = 10) consisted solely of blood-only and serum-only samples. The remaining 80 teeth were used for the experimental groups with four different medicaments. Three weeks later, either blood or PRF was applied as a scaffold after removing the medicaments (n = 10). Color changes were assessed before medication placement and at the end of the first, second, and third weeks, as well as on days 0, 1, 30, 60, and 90 after scaffold application. Analysis was carried out using repeated measures of variance, Friedman, one-way ANOVA, Kruskal-Wallis, the dependent paired t-test, and Wilcoxon test.
RESULTS: Statistical significance was determined at P = 0.05. All groups including blood and the group including propolis and PRF combination, resulted in a significant increase in discoloration (P < 0.05) and discoloration exceeding clinically acceptable thresholds.
CONCLUSIONS: CH and the modified versions of TAP (mTAPd) and DAP (mDAP) demonstrated an acceptable level of discoloration when used with a combination of PRF at day 90.
OBJECTIVE: This study aimed to investigate the combined crown discoloration of scaffolds [platelet-rich fibrin (PRF) and blood clot] applied after administration of different medicaments [modified triple antibiotic paste including doxycycline (mTAPd), modified double antibiotic paste (mDAP), calcium hydroxide (CH), and propolis].
METHODS: In total, 100 human mandibular premolar teeth were selected and prepared. The teeth were apically resected to simulate immature teeth. The positive and negative control groups (n = 10) consisted solely of blood-only and serum-only samples. The remaining 80 teeth were used for the experimental groups with four different medicaments. Three weeks later, either blood or PRF was applied as a scaffold after removing the medicaments (n = 10). Color changes were assessed before medication placement and at the end of the first, second, and third weeks, as well as on days 0, 1, 30, 60, and 90 after scaffold application. Analysis was carried out using repeated measures of variance, Friedman, one-way ANOVA, Kruskal-Wallis, the dependent paired t-test, and Wilcoxon test.
RESULTS: Statistical significance was determined at P = 0.05. All groups including blood and the group including propolis and PRF combination, resulted in a significant increase in discoloration (P < 0.05) and discoloration exceeding clinically acceptable thresholds.
CONCLUSIONS: CH and the modified versions of TAP (mTAPd) and DAP (mDAP) demonstrated an acceptable level of discoloration when used with a combination of PRF at day 90.
摘要：
背景：再生牙髓涉及使用各种根管药物和支架，这可能会导致表冠变色。
目的：本研究旨在研究不同药物给药后支架[富血小板纤维蛋白（PRF）和血凝块]的联合冠变色[改良三联抗生素糊剂包括多西环素（mTAPd），改良双抗生素糊剂（mDAP），氢氧化钙(CH),和蜂胶]。
方法：总共，选择并制备100颗人类下颌前磨牙。对牙齿进行顶端切除以模拟未成熟的牙齿。阳性和阴性对照组(n=10)仅由仅血液和仅血清样品组成。其余80颗牙齿用于具有四种不同药物的实验组。三周后,在除去药物后,将血液或PRF作为支架应用(n=10)。在药物放置之前和第一次结束时评估颜色变化，第二,第三周,以及在支架应用后的第0、1、30、60和90天。使用重复的方差测量进行分析，弗里德曼,单向方差分析,Kruskal-Wallis,依赖配对t检验，和Wilcoxon测试.
结果:在P=0.05时确定统计学显著性。所有组包括血液和该组包括蜂胶和PRF组合，导致变色显着增加（P<0.05），变色超过临床可接受的阈值。
结论：CH和TAP（mTAPd）和DAP（mDAP）的改良版本在第90天与PRF组合使用时显示出可接受的变色水平。
目的：本研究旨在研究不同药物给药后支架[富血小板纤维蛋白（PRF）和血凝块]的联合冠变色[改良三联抗生素糊剂包括多西环素（mTAPd），改良双抗生素糊剂（mDAP），氢氧化钙(CH),和蜂胶]。
方法：总共，选择并制备100颗人类下颌前磨牙。对牙齿进行顶端切除以模拟未成熟的牙齿。阳性和阴性对照组(n=10)仅由仅血液和仅血清样品组成。其余80颗牙齿用于具有四种不同药物的实验组。三周后,在除去药物后,将血液或PRF作为支架应用(n=10)。在药物放置之前和第一次结束时评估颜色变化，第二,第三周,以及在支架应用后的第0、1、30、60和90天。使用重复的方差测量进行分析，弗里德曼,单向方差分析,Kruskal-Wallis,依赖配对t检验，和Wilcoxon测试.
结果:在P=0.05时确定统计学显著性。所有组包括血液和该组包括蜂胶和PRF组合，导致变色显着增加（P<0.05），变色超过临床可接受的阈值。
结论：CH和TAP（mTAPd）和DAP（mDAP）的改良版本在第90天与PRF组合使用时显示出可接受的变色水平。
