PDF(Pubmed)
Abstract：
The Eaf6 protein, a conserved component of the NuA4 and NuA3 complexes in yeast and MOZ/MORF complexes in humans, plays crucial roles in transcriptional activation, gene regulation, and cell cycle control. Despite its significance in other organisms, the functional role of Eaf6 in entomopathogenic fungi (EPF) remained unexplored. Here, we investigate the function of BbEaf6, the Eaf6 homolog in the entomopathogenic fungus Beauveria bassiana. We demonstrate that BbEaf6 is predominantly localized in nuclei, similar to its counterpart in other fungi. Deletion of BbEaf6 resulted in delayed conidiation, reduced conidial yield, and altered conidial properties. Transcriptomic analysis revealed dysregulation of the genes involved in asexual development and cell cycle progression in the ΔBbEaf6 mutant. Furthermore, the ΔBbEaf6 mutant exhibited decreased tolerance to various stresses, including ionic stress, cell wall perturbation, and DNA damage stress. Notably, the ΔBbEaf6 mutant displayed attenuated virulence in insect bioassays, accompanied by dysregulation of genes associated with cuticle penetration and haemocoel infection. Overall, our study elucidates the multifaceted role of BbEaf6 in stress response, development, and virulence in B. bassiana, providing valuable insights into the molecular mechanisms governing fungal pathogenesis and potential targets for pest management strategies.
摘要：
Eaf6蛋白，酵母和人类MOZ/MORF复合物中NuA4和NuA3复合物的保守成分，在转录激活中起着至关重要的作用，基因调控，和细胞周期控制。尽管它在其他生物体中具有重要意义，Eaf6在昆虫病原真菌（EPF）中的功能作用仍未被探索。这里,我们研究了Eaf6同源物在昆虫病原真菌球孢白僵菌中的功能。我们证明BbEaf6主要位于细胞核中，与其他真菌类似。BbEaf6的删除导致延迟的分生孢子，分生孢子产量降低，并改变了分生孢子的性质。转录组学分析显示，ΔBbEaf6突变体中涉及无性发育和细胞周期进程的基因失调。此外，ΔBbEaf6突变体对各种胁迫的耐受性降低，包括离子应力，细胞壁扰动，和DNA损伤压力。值得注意的是,ΔBbEaf6突变体在昆虫生物测定中显示出减弱的毒力，伴随着与角质层渗透和血球感染相关的基因失调。总的来说,我们的研究阐明了BbEaf6在应激反应中的多方面作用，发展,和B.bassiana的毒力，为控制真菌发病机理的分子机制和害虫管理策略的潜在目标提供有价值的见解。
