METHODS: This cross-sectional study involved subjects (n = 60) diagnosed with stage III grade B-C (n = 13) to stage IV grade C (n = 17) periodontitis, and periodontally healthy controls (n = 30). Clinical periodontal measurements involved full-mouth. The concentrations of IL-10, IL-12, and IL-18 were determined using enzyme-linked immunosorbent assay (ELISA).
RESULTS: There were no significant differences in IL-12 level and IL-18/IL-10 ratio between the healthy and periodontitis groups (p = 0.413, p = 0.636, respectively). The IL-10 and IL-18 levels were significantly higher in the periodontitis group than in controls (p < 0.001, p < 0.001, respectively). Significant associations were observed between the periodontitis and IL-10 and IL-18 levels (OR = 1.46, %95 CI 1.19-1.795; OR = 1.13, %95 CI 1.059-1.207, respectively) (p < 0.001, p < 0.001, respectively).
CONCLUSIONS: There was a correlation between pocket depth and the presence of IL-18 and a strong association between periodontitis and a high level of IL-18. However, there were no direct correlations among the three biomarkers and IL-18/IL-10 ratio, indicating that their roles in periodontal health are complex and multidimensional.
CONCLUSIONS: Understanding the cytokine dynamics in GCF provides valuable insights into their potential clinical implications for periodontal disease diagnosis, risk assessment, and tailored therapeutic interventions.
方法:这项横断面研究涉及被诊断为III期B-C级(n=13)至IV期C级(n=17)牙周炎的受试者(n=60),和牙周健康对照(n=30)。临床牙周测量涉及全口。使用酶联免疫吸附测定(ELISA)测定IL-10、IL-12和IL-18的浓度。
结果:健康组和牙周炎组之间的IL-12水平和IL-18/IL-10比率没有显着差异(分别为p=0.413,p=0.636)。牙周炎组的IL-10和IL-18水平明显高于对照组(分别为p<0.001,p<0.001)。观察到牙周炎与IL-10和IL-18水平之间存在显着相关性(分别为OR=1.46,%95CI1.19-1.795;OR=1.13,%95CI1.059-1.207)(分别为p<0.001,p<0.001)。
结论:囊袋深度与IL-18的存在之间存在相关性,牙周炎与高水平IL-18之间存在很强的相关性。然而,三种生物标志物与IL-18/IL-10比值之间没有直接相关性,表明它们在牙周健康中的作用是复杂和多维的。
结论:了解GCF中的细胞因子动力学为其对牙周病诊断的潜在临床意义提供了有价值的见解。风险评估,和量身定制的治疗干预措施。