PDF(Pubmed)
Abstract：
Endovascular middle cerebral artery occlusion (MCAO) is a widely used experimental ischemic stroke model. However, the model carries high early mortality. Our aim was to investigate the factors that influence early mortality within 48 h of reperfusion after transient MCAO. Using C57BL/6 mice, we induced 1-hour endovascular filament MCAO. To introduce heterogeneity of infarct volumes, a subset of animals had additional tandem common carotid artery occlusion (MCAO+CCAO). Continuous video monitoring was used to gain insight into the cause of death. Mortality within 48 h was 25% in the pooled cohort. All animals with early mortality suffered from infarcts in the hippocampus, sometimes accompanied by infarcts in the thalamus and midbrain, which occurred exclusively in the MCAO+CCAO group. All animals with early mortality developed convulsive seizures captured on video monitoring. None of the animals that did not develop convulsive seizures died. Among the three regions, hippocampal infarction appeared necessary for convulsive seizures and early mortality. Our data highlight seizures as the primary cause of mortality within the first 48 h after endovascular filament MCAO, linked to hippocampal infarction. Since hippocampal blood supply is mainly from the posterior cerebral artery (PCA), avoiding concurrent PCA ischemia can decrease mortality in proximal MCAO models.
摘要：
血管内大脑中动脉闭塞（MCAO）是一种广泛使用的实验性缺血性中风模型。然而,该模型具有很高的早期死亡率。我们的目的是研究短暂性MCAO后再灌注48小时内影响早期死亡率的因素。使用C57BL/6小鼠，我们诱导了1小时血管内细丝MCAO。为了引入梗死体积的异质性，一部分动物有额外的串联颈总动脉闭塞(MCAO+CCAO).连续视频监控用于了解死亡原因。在合并队列中，48小时内的死亡率为25%。所有早期死亡的动物都患有海马区梗塞，有时伴有丘脑和中脑梗塞，仅发生在MCAO+CCAO组。所有早期死亡的动物都发生了在视频监控中捕获的惊厥性癫痫发作。没有发生惊厥性癫痫发作的动物都没有死亡。在这三个地区中，海马梗死似乎是惊厥性癫痫发作和早期死亡所必需的。我们的数据强调癫痫发作是血管内细丝MCAO后最初48小时内死亡的主要原因，与海马梗死有关.由于海马血液供应主要来自大脑后动脉（PCA），避免并发PCA缺血可以降低近端MCAO模型的死亡率.
