Abstract：
There remains a lack of small-caliber tissue-engineered blood vessels (TEBVs) with wide clinical use. Biotubes were developed by electrospinning and in-body tissue architecture (iBTA) technology to prepare small-caliber TEBVs with promising applications. Different ratios of hybrid fibers of poly(l-lactic-co-ε-caprolactone) (PLCL) and polyurethane (PU) were obtained by electrospinning, and the electrospun tubes were then implanted subcutaneously in the abdominal area of a rabbit (as an in vivo bioreactor). The biotubes were harvested after 4 weeks. They were then decellularized and cross-linked with heparin. PLCL/PU electrospun vascular tubes, decellularized biotubes (D-biotubes), and heparinized combined decellularized biotubes (H + D-biotubes) underwent carotid artery allograft transplantation in a rabbit model. Vascular ultrasound follow-up and histological observation revealed that the biotubes developed based on electrospinning and iBTA technology, after decellularization and heparinization cross-linking, showed a better patency rate, adequate mechanical properties, and remodeling ability in the rabbit model. IBTA technology caused a higher patency, and the heparinization cross-linking process gave the biotubes stronger mechanical properties.
摘要：
仍然缺乏具有广泛临床用途的小口径组织工程血管（TEBV）。通过静电纺丝和体内组织结构（iBTA）技术开发了生物管，以制备具有广阔应用前景的小口径TEBV。通过静电纺丝获得不同比例的聚（1-乳酸-co-ε-己内酯）（PLCL）和聚氨酯（PU）的混合纤维，然后将电纺管皮下植入兔的腹部区域(作为体内生物反应器)。4周后收获生物管。然后将它们脱细胞并与肝素交联。PLCL/PU静电纺丝血管管，去细胞生物管（D-biotubes），和肝素化的联合脱细胞生物管（HD-生物管）在兔模型中进行了颈动脉同种异体移植。血管超声随访和组织学观察显示，基于静电纺丝和iBTA技术开发的生物管，脱细胞和肝素化交联后，显示出更好的通畅率，足够的机械性能，和兔模型中的重塑能力。IBTA技术造成了更高的通畅性，肝素化交联过程使生物管具有更强的机械性能。
