PDF(Pubmed)
Abstract：
The aggregation and spreading of \"tau-seeds\" are key for the development and progression of tauopathies, including Alzheimer\'s disease. Here we describe the steps to isolate and analyze biochemically active tau-seeds from human, mouse, and cell origin. We detail the procedure to isolate soluble tau-seeds by size exclusion chromatography and seeding assay. The isolated tau-seed can be further analyzed to determine the interactome by mass spectrometry. This workflow identifies protein-protein interactors of tau-seeds, providing a useful tool for finding new therapeutic targets. For complete details on the use and execution of this protocol, please refer to Martinez et al.1.
摘要：
“tau种子”的聚集和传播是tau蛋白病发展和进展的关键，包括老年痴呆症.在这里，我们描述了从人类中分离和分析生化活性tau种子的步骤，鼠标,细胞起源。我们详细介绍了通过尺寸排阻色谱和接种测定法分离可溶性tau种子的程序。可以进一步分析分离的tau-种子以通过质谱法确定相互作用组。这个工作流程识别了tau种子的蛋白质-蛋白质相互作用物，为寻找新的治疗靶点提供了有用的工具。有关此协议的使用和执行的完整详细信息，PleaserefertoMartinezetal.1.
