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Abstract：
Introduction Oxidative stress, an imbalance between reactive oxygen species (ROS) production and antioxidant defenses, plays an important role in various dental diseases. Local anesthetics are frequently used in dentistry. The potential antioxidant activity of dental local anesthetics can contribute to dental practice. Therefore, this study aimed to investigate the ROS-scavenging activities of three commonly used dental local anesthetics, lidocaine, prilocaine, and articaine, focusing on their effects on hydroxyl radicals (HO•) and superoxide anions (O2 •-). Materials and methods The electron spin resonance (ESR) spin-trapping technique was employed to specifically measure the ROS-scavenging activities of these local anesthetics at varying concentrations. Results Lidocaine, prilocaine, and articaine exhibited concentration-dependent HO•-scavenging activities, with IC50 values of 0.029%, 0.019%, and 0.014%, respectively. Lidocaine and prilocaine showed concentration-dependent O2 •--scavenging activity, with IC50 values of 0.033% and 0.057%, respectively. However, articaine did not scavenge O2 •-. Conclusions The proactive use of dental local anesthetics may mitigate oxidative injury and inflammatory damage through direct ROS scavenging. However, further research is needed to elucidate the specific mechanisms underlying the antioxidant effects of these dental local anesthetics and their potential impact on the dental diseases associated with oxidative stress.
摘要：
简介氧化应激，活性氧（ROS）产生和抗氧化防御之间的不平衡，在各种牙科疾病中起着重要作用。局部麻醉药经常用于牙科。牙科局部麻醉药的潜在抗氧化活性可有助于牙科实践。因此,本研究旨在探讨三种常用的牙科局部麻醉药的ROS清除活性,利多卡因,丙胺卡因,还有阿替卡因,重点研究它们对羟基自由基(HO•)和超氧阴离子(O2•-)的影响。材料和方法采用电子自旋共振（ESR）自旋捕获技术来专门测量不同浓度下这些局部麻醉剂的ROS清除活性。结果利多卡因,丙胺卡因,阿替卡因表现出浓度依赖性HO·-清除活性，IC50值为0.029%，0.019%,和0.014%，分别。利多卡因和丙胺卡因显示浓度依赖性O2•-清除活性，IC50值为0.033%和0.057%，分别。然而,阿替卡因未清除O2•-。结论积极使用局部麻醉药可通过直接清除ROS减轻氧化损伤和炎症损伤。然而,需要进一步的研究来阐明这些牙科局部麻醉药抗氧化作用的具体机制,以及它们对与氧化应激相关的牙科疾病的潜在影响.
