Five Sprague-Dawley rats (age 8-9 weeks, male) underwent double lacrimal gland removal. Modified Schirmer\'s tear test, blink tests, and in vivo confocal microscopy images were acquired pre-surgery and at 1, 2, and 4 weeks post-surgery. Three individual stromal nerves were selected per eye as guide images, and images of the corresponding sub-basal nerve plexus area were acquired via volume acquisition. The same area was re-imaged in subsequent weeks.
After double lacrimal gland removal, tear production was reduced by 60%, and the blink rate increased 10 times compared to pre-surgery. Starting from 1 week after surgery, in vivo confocal microscopy showed increased sub-basal nerve plexus nerve fiber density with inflammatory cell infiltration at the sub-basal nerve plexus layer and remained at an elevated level at 2 and 4 weeks post-surgery.
We demonstrated that our precise monitoring method revealed detailed changes in the corneal nerves, the epithelium, and the stroma.
■五只Sprague-Dawley大鼠(8-9周龄,男性)接受了双泪腺切除术。改良Schirmer撕裂试验,眨眼测试,在术前和术后1、2和4周获得体内共聚焦显微镜图像。每只眼睛选择三个单独的基质神经作为引导图像,通过体积采集获得相应的基底下神经丛区域的图像。在随后的几周内对相同区域重新成像。
■双泪腺切除后,泪液产量减少了60%,与手术前相比,眨眼率增加了10倍。从手术后1周开始,体内共聚焦显微镜显示基底神经丛下神经纤维密度增加,基底神经丛下层有炎性细胞浸润,术后2周和4周仍处于升高水平.
■我们证明了我们的精确监测方法揭示了角膜神经的详细变化,上皮,和基质。