PDF(Pubmed)
Abstract：
While lacrimal gland removal is commonly used in animal models to replicate dry eye disease, research into systematically monitoring dry eye disease\'s longitudinal pathological changes is limited. In vivo confocal microscopy (Heidelberg Retina Tomograph 3 with a Rostock Cornea Module, Heidelberg Engineering Inc., Franklin, MA) can non-invasively reveal corneal histopathological structures. To monitor dry-eye-disease-related changes in corneal structures, we developed a precise monitoring method using in vivo confocal microscopy in a rat double lacrimal gland removal model.
Five Sprague-Dawley rats (age 8-9 weeks, male) underwent double lacrimal gland removal. Modified Schirmer\'s tear test, blink tests, and in vivo confocal microscopy images were acquired pre-surgery and at 1, 2, and 4 weeks post-surgery. Three individual stromal nerves were selected per eye as guide images, and images of the corresponding sub-basal nerve plexus area were acquired via volume acquisition. The same area was re-imaged in subsequent weeks.
After double lacrimal gland removal, tear production was reduced by 60%, and the blink rate increased 10 times compared to pre-surgery. Starting from 1 week after surgery, in vivo confocal microscopy showed increased sub-basal nerve plexus nerve fiber density with inflammatory cell infiltration at the sub-basal nerve plexus layer and remained at an elevated level at 2 and 4 weeks post-surgery.
We demonstrated that our precise monitoring method revealed detailed changes in the corneal nerves, the epithelium, and the stroma.
Five Sprague-Dawley rats (age 8-9 weeks, male) underwent double lacrimal gland removal. Modified Schirmer\'s tear test, blink tests, and in vivo confocal microscopy images were acquired pre-surgery and at 1, 2, and 4 weeks post-surgery. Three individual stromal nerves were selected per eye as guide images, and images of the corresponding sub-basal nerve plexus area were acquired via volume acquisition. The same area was re-imaged in subsequent weeks.
After double lacrimal gland removal, tear production was reduced by 60%, and the blink rate increased 10 times compared to pre-surgery. Starting from 1 week after surgery, in vivo confocal microscopy showed increased sub-basal nerve plexus nerve fiber density with inflammatory cell infiltration at the sub-basal nerve plexus layer and remained at an elevated level at 2 and 4 weeks post-surgery.
We demonstrated that our precise monitoring method revealed detailed changes in the corneal nerves, the epithelium, and the stroma.
摘要：
■虽然泪腺切除通常在动物模型中用于复制干眼症，系统监测干眼病纵向病理变化的研究是有限的。体内共聚焦显微镜（带有罗斯托克角膜模块的海德堡视网膜断层扫描3，海德堡工程公司富兰克林MA）可以非侵入性地显示角膜组织病理学结构。监测干眼症相关的角膜结构变化，我们在大鼠双泪腺切除模型中使用体内共聚焦显微镜开发了一种精确的监测方法。
■五只Sprague-Dawley大鼠（8-9周龄，男性)接受了双泪腺切除术。改良Schirmer撕裂试验,眨眼测试,在术前和术后1、2和4周获得体内共聚焦显微镜图像。每只眼睛选择三个单独的基质神经作为引导图像，通过体积采集获得相应的基底下神经丛区域的图像。在随后的几周内对相同区域重新成像。
■双泪腺切除后，泪液产量减少了60%,与手术前相比，眨眼率增加了10倍。从手术后1周开始，体内共聚焦显微镜显示基底神经丛下神经纤维密度增加,基底神经丛下层有炎性细胞浸润,术后2周和4周仍处于升高水平.
■我们证明了我们的精确监测方法揭示了角膜神经的详细变化，上皮，和基质。
■五只Sprague-Dawley大鼠（8-9周龄，男性)接受了双泪腺切除术。改良Schirmer撕裂试验,眨眼测试,在术前和术后1、2和4周获得体内共聚焦显微镜图像。每只眼睛选择三个单独的基质神经作为引导图像，通过体积采集获得相应的基底下神经丛区域的图像。在随后的几周内对相同区域重新成像。
■双泪腺切除后，泪液产量减少了60%,与手术前相比，眨眼率增加了10倍。从手术后1周开始，体内共聚焦显微镜显示基底神经丛下神经纤维密度增加,基底神经丛下层有炎性细胞浸润,术后2周和4周仍处于升高水平.
■我们证明了我们的精确监测方法揭示了角膜神经的详细变化，上皮，和基质。
