PDF(Pubmed)
Abstract：
UNASSIGNED: Ultrasonic (US) cutting of cartilage in orthopaedic surgery has received little attention despite its potential to reduce chondrocyte death which could enhance cartilage repair. We aimed to investigate whether an ultrasonically-vibrating scalpel to cut human articular cartilage could reduce chondrocyte death, and to determine if hyper-osmolarity could provide chondroprotection during the procedure.
UNASSIGNED: A scalpel (no. 15) was mounted on an ultrasonic transducer to resonate at 35 kHz with 30 μm vibrational displacement. Thirty-six fresh human femoral cartilage samples were divided into four groups based on ultrasonic activation (US or non-US) and saline osmolarity (300 or 600 mOsm/L). Cell viability was assessed using a live/dead cell assay and analysed quantitatively by confocal microscopy. Histology illustrated tissue surface changes at the cut site.
UNASSIGNED: The overall chondrocyte death percentage at both the US and non-US cut sites showed comparable results (p > 0.05) in both osmolarities. However, the zone of chondrocyte death was reduced by 31 ± 5% and 36 ± 6%, respectively, when comparing US cutting at 300 mOsm/L and 600 mOsm/L to the control group (non-US cutting; 300 mOsm/L) (p < 0.05). The width of the cut was consistent at both sites, regardless of the method of cutting.
UNASSIGNED: Cutting human cartilage with US in the presence of 300 or 600 mOsm/L media was chondroprotective compared to normal (non-US) scalpel cutting in 300 mOsm/L medium. These results suggest chondroprotection can be achieved while cutting using a US scalpel and raised osmolarity, potentially improving cartilage regeneration and repair following injury.
UNASSIGNED: A scalpel (no. 15) was mounted on an ultrasonic transducer to resonate at 35 kHz with 30 μm vibrational displacement. Thirty-six fresh human femoral cartilage samples were divided into four groups based on ultrasonic activation (US or non-US) and saline osmolarity (300 or 600 mOsm/L). Cell viability was assessed using a live/dead cell assay and analysed quantitatively by confocal microscopy. Histology illustrated tissue surface changes at the cut site.
UNASSIGNED: The overall chondrocyte death percentage at both the US and non-US cut sites showed comparable results (p > 0.05) in both osmolarities. However, the zone of chondrocyte death was reduced by 31 ± 5% and 36 ± 6%, respectively, when comparing US cutting at 300 mOsm/L and 600 mOsm/L to the control group (non-US cutting; 300 mOsm/L) (p < 0.05). The width of the cut was consistent at both sites, regardless of the method of cutting.
UNASSIGNED: Cutting human cartilage with US in the presence of 300 or 600 mOsm/L media was chondroprotective compared to normal (non-US) scalpel cutting in 300 mOsm/L medium. These results suggest chondroprotection can be achieved while cutting using a US scalpel and raised osmolarity, potentially improving cartilage regeneration and repair following injury.
摘要：
■骨科手术中软骨的超声（US）切割很少受到关注，尽管它具有减少软骨细胞死亡的潜力，可以增强软骨修复。我们旨在研究超声振动手术刀切割人关节软骨是否可以减少软骨细胞死亡，并确定高渗透压是否可以在手术过程中提供软骨保护。
■手术刀（编号：15)安装在超声换能器上，以35kHz共振，振动位移为30μm。根据超声激活（US或非US）和盐水渗透压（300或600mOsm/L），将36个新鲜的人股软骨样品分为四组。使用活/死细胞测定评估细胞活力,并通过共聚焦显微镜定量分析。组织学说明了切割部位的组织表面变化。
■美国和非美国切割位点的整体软骨细胞死亡百分比在两种渗透压下均显示出可比的结果（p>0.05）。然而,软骨细胞死亡区域减少了31±5%和36±6%，分别,将300mOsm/L和600mOsm/L的US切割与对照组(非US切割；300mOsm/L)进行比较(p<0.05)。两个地点的切口宽度一致，不管切割的方法。
在300或600mOsm/L培养基存在下用US切割人软骨与在300mOsm/L培养基中切割正常(非US)手术刀相比具有软骨保护作用。这些结果表明，使用美国手术刀进行切割并提高渗透压可以实现软骨保护，有可能改善损伤后的软骨再生和修复。
■手术刀（编号：15)安装在超声换能器上，以35kHz共振，振动位移为30μm。根据超声激活（US或非US）和盐水渗透压（300或600mOsm/L），将36个新鲜的人股软骨样品分为四组。使用活/死细胞测定评估细胞活力,并通过共聚焦显微镜定量分析。组织学说明了切割部位的组织表面变化。
■美国和非美国切割位点的整体软骨细胞死亡百分比在两种渗透压下均显示出可比的结果（p>0.05）。然而,软骨细胞死亡区域减少了31±5%和36±6%，分别,将300mOsm/L和600mOsm/L的US切割与对照组(非US切割；300mOsm/L)进行比较(p<0.05)。两个地点的切口宽度一致，不管切割的方法。
在300或600mOsm/L培养基存在下用US切割人软骨与在300mOsm/L培养基中切割正常(非US)手术刀相比具有软骨保护作用。这些结果表明，使用美国手术刀进行切割并提高渗透压可以实现软骨保护，有可能改善损伤后的软骨再生和修复。
