PDF(Pubmed)
Abstract：
OBJECTIVE: Dietary proteins are broken down into peptides across the gastrointestinal tract, with skeletal muscle being a primary deposition site for amino acids in the form of incorporation into, for example, metabolic and structural proteins. It follows that key research questions remain as to the role of amino acid bioavailability, of which protein digestibility and splanchnic sequestration (absorption and utilization) of amino acids are determining factors, impact upon muscle protein synthesis (MPS) in clinical states.
RESULTS: Elevated splanchnic amino acid uptake has been implicated in anabolic resistance (i.e. attenuated anabolic responses to protein intake) observed in ageing, though it is unclear whether this limits MPS. The novel \'dual stable isotope tracer technique\' offers a promising, minimally invasive approach to quantify the digestion of any protein source(s). Current work is focused on the validation of this technique against established methods, with scope to apply this to clinical and elderly populations to help inform mechanistic and interventional insights.
CONCLUSIONS: Considerations should be made for all facets of protein quality; digestibility of the protein, absorption/utilization and subsequent peripheral bioavailability of amino acids, and resultant stimulation of MPS. Stable isotope tracer techniques offer a minimally invasive approach to achieve this, with wide-ranging clinical application.
RESULTS: Elevated splanchnic amino acid uptake has been implicated in anabolic resistance (i.e. attenuated anabolic responses to protein intake) observed in ageing, though it is unclear whether this limits MPS. The novel \'dual stable isotope tracer technique\' offers a promising, minimally invasive approach to quantify the digestion of any protein source(s). Current work is focused on the validation of this technique against established methods, with scope to apply this to clinical and elderly populations to help inform mechanistic and interventional insights.
CONCLUSIONS: Considerations should be made for all facets of protein quality; digestibility of the protein, absorption/utilization and subsequent peripheral bioavailability of amino acids, and resultant stimulation of MPS. Stable isotope tracer techniques offer a minimally invasive approach to achieve this, with wide-ranging clinical application.
摘要：
目的：膳食蛋白质通过胃肠道分解成肽，骨骼肌是氨基酸的主要沉积位点，例如,代谢和结构蛋白。因此，关键的研究问题仍然是氨基酸生物利用度的作用，其中蛋白质消化率和内脏螯合(吸收和利用)氨基酸是决定因素，对临床状态下肌肉蛋白合成（MPS）的影响。
结果：内脏氨基酸摄取升高与衰老中观察到的合成代谢抗性（即对蛋白质摄入的合成代谢反应减弱）有关，尽管目前尚不清楚这是否限制了MPS。新型的“双稳定同位素示踪技术”提供了一种有前途的，微创方法来量化任何蛋白质来源的消化。当前的工作集中在针对已建立的方法验证这种技术，可以将其应用于临床和老年人群，以帮助告知机械和干预见解。
结论：应考虑蛋白质质量的所有方面；蛋白质的消化率，氨基酸的吸收/利用和随后的外周生物利用度，以及由此产生的MPS刺激。稳定同位素示踪技术提供了一种微创方法来实现这一目标，具有广泛的临床应用。
结果：内脏氨基酸摄取升高与衰老中观察到的合成代谢抗性（即对蛋白质摄入的合成代谢反应减弱）有关，尽管目前尚不清楚这是否限制了MPS。新型的“双稳定同位素示踪技术”提供了一种有前途的，微创方法来量化任何蛋白质来源的消化。当前的工作集中在针对已建立的方法验证这种技术，可以将其应用于临床和老年人群，以帮助告知机械和干预见解。
结论：应考虑蛋白质质量的所有方面；蛋白质的消化率，氨基酸的吸收/利用和随后的外周生物利用度，以及由此产生的MPS刺激。稳定同位素示踪技术提供了一种微创方法来实现这一目标，具有广泛的临床应用。
