Abstract：
Melanoma is one of the most aggressive and fatal skin cancers owing to its ability to metastasize and develop resistance to chemotherapy. Photodynamic therapy (PDT) is a minimally noninvasive treatment modality comprising photosensitizers (PSs), light sources, and endogenous molecular oxygen that exert a localized cytotoxic effect on cancer cells. The current study explores the therapeutic potential of sodium copper chlorophyllin-loaded chitosan nanoparticles (CH-SCC NPs) along with handheld laser-based PDT on B16 cancer cells. A modified chlorophyll derivative identified as sodium copper chlorophyllin (SCC) is a dietary supplement that has anticancer properties. Herein, we have synthesized CH-SCC NPs using the ionic gelation method to enhance the PS\'s bioavailability and efficiency. Chitosan nanoparticles exhibited high biocompatibility in a normal cell line L929, zebrafish, and chick embryos, and were successfully employed to deliver the SCC to cancer cells. CH-SCC NPs showed an enhanced PDT effect that killed approximately 80%-85% of B16 cells. CH-SCC NPs in combination with a handheld portable laser source showed significant therapeutic potential against the B16 skin cancer cell line. The experimental findings further strengthen our device-repurposing strategy, which suggests that SCC nanoformulations along with handheld laser can be a suitable treatment for skin cancer even in remote areas where power source and treatment cost can be a limitation.
摘要：
黑色素瘤是最具侵袭性和致命性的皮肤癌之一，因为它具有转移和对化疗产生抗性的能力。光动力疗法（PDT）是一种微创治疗方式，包括光敏剂（PS），光源,和内源性分子氧，对癌细胞产生局部细胞毒性作用。目前的研究探索了装载叶绿素铜钠的壳聚糖纳米颗粒（CH-SCCNP）以及手持式激光PDT对B16癌细胞的治疗潜力。鉴定为叶绿素铜钠(SCC)的修饰的叶绿素衍生物是具有抗癌性质的膳食补充剂。在这里，我们使用离子凝胶法合成了CH-SCCNP，以提高PS的生物利用度和效率。壳聚糖纳米颗粒在正常细胞系L929、斑马鱼、和小鸡胚胎，并成功用于将SCC递送至癌细胞。CH-SCCNPs显示增强的PDT效应,其杀死约80%-85%的B16细胞。CH-SCCNP与手持式便携式激光源组合显示出针对B16皮肤癌细胞系的显着治疗潜力。实验结果进一步加强了我们的设备再利用策略，这表明SCC纳米制剂与手持式激光一起可以是皮肤癌的合适治疗方法，即使在电源和治疗成本有限的偏远地区也是如此。
