RESULTS: This study, employing 16S rRNA sequencing, demonstrates distinct shifts in the larvae gut microbiome of C. punctiferalis between different feeding stages, highlighting a pronounced diversity in the early-instar with Enterococcus as a predominant genus in laboratory populations. Through in vitro cultivation and sequencing, three bacterial strains - Micrococcus sp., Brevibacterium sp. and Enterococcus mundtii - were isolated and characterized. Bioassays revealed that E. mundtii-infused corn significantly boosts early-instar larval growth, enhancing both body length and weight. Quantitative PCR and spectrophotometry confirmed a higher abundance of E. mundtii in younger larvae, correlating with increased digestive enzyme activity and total protein levels.
CONCLUSIONS: This study reveals the heightened gut microbiota diversity in early instars of C. punctiferalis larvae, highlighting that Enterococcus represent a predominant bacteria in laboratory populations. In vitro cultivation and bioassays unequivocally demonstrate the significant role of the cultivable gut bacteria E. mundtii in promoting the growth of early-instar larva. These findings provide a solid theoretical foundation for advancing the comprehension of the intricate interactions between gut microbiota and insect hosts, as well as for the development of eco-friendly pest control technologies based on targeted manipulation of insect gut microbial communities. © 2024 Society of Chemical Industry.
结果:这项研究,采用16SrRNA测序,显示了不同喂养阶段之间点状芽孢杆菌幼虫肠道微生物组的不同变化,突出了早龄的显着多样性,其中肠球菌是实验室种群中的主要属。通过体外培养和测序,三种细菌菌株-微球菌。,短杆菌属。和门肠球菌-被分离和表征。生物测定显示,注入E.mundtii的玉米显着促进早熟幼虫的生长,增强身长和体重。定量PCR和分光光度法证实了较年轻的幼虫中E.mundtii的丰度较高,与消化酶活性和总蛋白质水平增加有关。
结论:这项研究揭示了点状芽孢杆菌幼虫早期幼虫肠道菌群多样性的增加,强调肠球菌是实验室人群中的主要细菌。体外培养和生物测定明确证明了可培养的肠道细菌E.mundtii在促进早龄幼虫生长中的重要作用。这些发现为推进肠道微生物群与昆虫宿主之间复杂相互作用的理解提供了坚实的理论基础。以及基于昆虫肠道微生物群落的针对性操纵的生态友好型害虫控制技术的发展。©2024化学工业学会。