PDF(Pubmed)
Abstract：
Traumatic and postoperative hemorrhages are life-threatening complications. Ankaferd BloodStopper (ABS) is a potent topical hemostatic agent to stop bleeding. However, ABS is associated with nerve toxicity. The present study aimed to investigate the functional and structural neurodegenerative effects of ABS in a mouse model. A total of 30 male BALB/c mice, aged 6-8 weeks, were randomly divided into control group (no treatment), a sham group (treated with saline) and an experimental group (treated with ABS). In the saline and the ABS groups, the right sciatic nerve was surgically exposed and treated with saline or ABS, respectively. No surgical procedure was performed in the control group. On day 7 post-treatment, functional changes of the sciatic nerve were evaluated by a horizontal ladder rung walking task. Structural changes were assessed with immunohistochemistry. In the horizontal ladder rung walking test, the gait impairment was proportional to the severity of sciatic nerve damage, with the ABS group showing a significantly higher rate of errors than the control and saline groups. Immunohistochemistry demonstrated extensive degeneration and deformation in the axons and myelin sheath of the sciatic nerve in the ABS group. The results provide compelling evidence for the neurotoxicity of ABS.
摘要：
创伤和术后出血是危及生命的并发症。AnkaferdBloodstopper（ABS）是一种有效的局部止血剂，可以止血。然而,ABS与神经毒性有关。本研究旨在研究ABS在小鼠模型中的功能和结构神经变性作用。共30只雄性BALB/c小鼠，年龄6-8周，随机分为对照组（不治疗），假手术组(用盐水处理)和实验组(用ABS处理)。在生理盐水和ABS组中，右侧坐骨神经经手术暴露，用生理盐水或ABS治疗，分别。对照组不进行外科手术。治疗后第7天，坐骨神经的功能变化通过水平梯级步行任务进行评估。用免疫组织化学评估结构变化。在水平梯子横档行走测试中，步态损伤与坐骨神经损伤的严重程度成正比，ABS组的错误率明显高于对照组和盐水组。免疫组织化学显示ABS组坐骨神经轴突和髓鞘广泛变性和变形。该结果为ABS的神经毒性提供了令人信服的证据。
