PDF(Pubmed)
Abstract：
Inflammatory illnesses, such as periodontitis and atherosclerotic coronary heart disease (ASCHD), trigger the production of pro-inflammatory mediators. The aim of this study was to assess the accuracy of using salivary interleukin-1β (IL-1β), interleukin-18 (IL-18), and gasdermin D (GSDMD) in discerning patients with periodontitis with and without ASCHD from healthy individuals, and to assess their correlation with clinical periodontal parameters and low-density lipoprotein (LDL) levels. The study involved 120 participants: 30 were healthy subjects (control group, C), 30 had generalized periodontitis (group P), 30 had ASCHD and clinically healthy periodontium (group AS-C), and 30 had ASCHD and generalized periodontitis (group AS-P). Saliva and blood samples were collected, and periodontal characteristics such as plaque index, bleeding on probing, probing pocket depth, and clinical attachment loss were examined. IL-1β, IL-18, and GSDMD levels from saliva were determined using ELISA. LDL levels were determined from the blood samples. Groups P, AS-C, and AS-P had higher levels of salivary IL-1β, IL-18, and GSDMD than group C. The receiver operating characteristic (ROC) curves of all biomarkers showed high diagnostic accuracy, with a significant positive correlation with the clinical parameters and LDL levels. The observed correlations between the studied pro-inflammatory mediators and disease severity suggest that these biomarkers could serve as indicators of disease progression in conditions such as periodontitis and ASCHD.
摘要：
炎症性疾病,如牙周炎和动脉粥样硬化性冠心病（ASCHD），引发促炎介质的产生。这项研究的目的是评估使用唾液白细胞介素-1β（IL-1β）的准确性，白细胞介素-18（IL-18），和gasderminD（GSDMD）从健康个体中辨别患有和不患有ASCHD的牙周炎患者，并评估其与临床牙周参数和低密度脂蛋白（LDL）水平的相关性。该研究涉及120名参与者：30名是健康受试者(对照组，C),30例广泛性牙周炎（P组），30例患者有ASCHD和临床健康的牙周病（AS-C组），30例患有ASCHD和全身性牙周炎（AS-P组）。收集唾液和血液样本，和牙周特征，如菌斑指数，探查时出血,探测袋深度,并检查了临床附着丧失。IL-1β,使用ELISA测定来自唾液的IL-18和GSDMD水平。从血液样品中测定LDL水平。P组,AS-C,AS-P有较高水平的唾液IL-1β，IL-18和GSDMD高于C组。所有生物标志物的受试者工作特征（ROC）曲线显示出较高的诊断准确性，与临床参数和LDL水平呈显著正相关。所研究的促炎介质与疾病严重程度之间观察到的相关性表明，这些生物标志物可以作为牙周炎和ASCHD等疾病进展的指标。
