UNASSIGNED: Heparin was passively adsorbed or carbodiimide-conjugated to microthreads, creating a biomimetic binding strategy, mimicking FGF2 sequestration in the extracellular matrix (ECM). It was also evaluated whether FGF2 incorporated into fibrin microthreads would yield sustained release. It was hypothesized that heparin-conjugated and co-incorporated (co-inc) fibrin microthreads would facilitate sustained release of FGF2 from the scaffold and enhance in vitro myoblast proliferation and outgrowth.
UNASSIGNED: Toluidine blue staining and Fourier transform infrared spectroscopy confirmed that carbodiimide-conjugated heparin bound to fibrin microthreads in a dose-dependent manner. Release kinetics revealed that heparin-conjugated fibrin microthreads exhibited sustained release of FGF2 over a period of one week. An in vitro assay demonstrated that FGF2 released from microthreads remained bioactive, stimulating myoblast proliferation over four days. Finally, a cellular outgrowth assay suggests that FGF2 promotes increased outgrowth onto microthreads.
UNASSIGNED: It was anticipated that the combined effects of fibrin microthread structural properties, topographic alignment cues, and FGF2 release profiles will facilitate the fabrication of a biomimetic scaffold that enhances the regeneration of functional muscle tissue for the treatment of VML injuries.
■肝素被动吸附或碳二亚胺缀合到微线上,创建仿生结合策略,模拟FGF2在细胞外基质(ECM)中的隔离。还评估了掺入纤维蛋白微线中的FGF2是否会产生持续释放。假设肝素缀合和共掺入(co-inc)的纤维蛋白微丝将促进FGF2从支架的持续释放并增强体外成肌细胞增殖和生长。
■甲苯胺蓝染色和傅里叶变换红外光谱证实,碳二亚胺缀合的肝素以剂量依赖性方式与纤维蛋白微丝结合。释放动力学显示肝素缀合的纤维蛋白微线在一周的时间内表现出FGF2的持续释放。体外试验表明,从微线释放的FGF2保持生物活性,刺激成肌细胞增殖超过四天。最后,细胞生长测定表明FGF2促进增加的生长到微线上。
■预期纤维蛋白微丝结构特性的综合作用,地形对齐提示,和FGF2释放曲线将促进仿生支架的制造,所述仿生支架增强用于治疗VML损伤的功能性肌肉组织的再生。