PDF(Pubmed)
Abstract：
Wild boars can directly or indirectly transmit numerous zoonotic helminths to humans in rural communities as they serve as reservoir hosts. This study was conducted to determine the occurrence and molecular characterisation of cystic metacestode forms of helminth parasites in wild boar and to identify haplotypes. To this end, 23 wild boar carcasses shot by hunters during the 2023 hunting season were necropsied and all internal organs were examined. Cysticercus tenuicollis (n = 07) and hydatid cyst (n = 10) isolates were obtained from the examined boars. Species identification of Cysticercus and hydatid cyst isolates was performed by amplification of partial fragments of the cox1 gene. According to BLAST search, all sequences of C. tenuicollis isolates were identified as Taenia hydatigena. Out of the hydatid cyst isolates, seven were classified as Echinococcus granulosus sensu stricto (G1/G3) and one sample was identified as Echinococcus canadensis (G6/G7). All isolates of E. granulosus s.s. (G1/G3) were re-amplified with the NADH dehydrogenase subunit 5 (nad5) in order to distinguish between G1 and G3 genotypes. Based on the sequence analysis, it was found that five of the E. granulosus s.s. isolates were classified as G1, while two were classified as G3. Based on the results of this study, it can be concluded that the G1 genotype is the most prevalent genetic variant among wild boar populations in Bingol province, Türkiye. In this study, a total of five novel haplotypes were identified. A previously unidentified haplotype was revealed through the haplotype analysis of E. canadensis (G6/G7). All isolates in the haplotype network of T. hydatigena were shown to exhibit distinct and geographically specific haplotypes. According to the findings of the study, wild boars include a substantial amount of genetic variety in E. granulosus s.s. And T. hydatigena.
摘要：
野猪作为水库宿主，可以直接或间接将许多人畜共患蠕虫传播给农村社区的人类。进行这项研究是为了确定野猪中蠕虫寄生虫的囊性节食形式的发生和分子特征，并鉴定单倍型。为此,对2023年狩猎季节中猎人拍摄的23只野猪尸体进行了尸检，并检查了所有内部器官。从检查的公猪中获得囊尾蚴（n=07）和包虫囊肿（n=10）的分离株。通过扩增cox1基因的部分片段进行囊尾蚴和包虫囊肿分离株的物种鉴定。根据BLAST搜索，Tenuicollis分离株的所有序列都被鉴定为Tu带虫。包虫囊肿分离株中，7个被归类为细粒棘球蚴（G1/G3），一个样本被鉴定为加拿大棘球蚴（G6/G7）。用NADH脱氢酶亚基5（nad5）重新扩增了所有细粒大肠杆菌（G1/G3）的分离株，以区分G1和G3基因型。根据序列分析，结果发现，5个S.S.分离株被分类为G1，而两个被分类为G3。根据这项研究的结果，可以得出结论，G1基因型是宾果省野猪种群中最普遍的遗传变异，蒂尔基耶.在这项研究中,共鉴定出5种新的单倍型.通过加拿大大肠杆菌(G6/G7)的单倍型分析揭示了以前未鉴定的单倍型。包虫病单倍型网络中的所有分离株都显示出不同的和地理上特定的单倍型。根据研究结果,野猪包括大量的遗传品种。
