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Abstract：
Drosophila border cell clusters model collective cell migration. Airyscan super-resolution microscopy enables fine-scale description of cluster shape and texture. Here we describe how to convert Airyscan images of border cell clusters into 3D models of the surface and detect regions of convex and concave curvature. We use spectral decomposition analysis to compare surface textures across genotypes to determine how genes of interest impact cluster surface geometry. This protocol applies to border cells and could generalize to additional cell types. For complete details on the use and execution of this protocol, please refer to Gabbert et al.1.
摘要：
果蝇边缘细胞簇模型集体细胞迁移。Airyscan超分辨率显微镜可以对簇的形状和纹理进行精细描述。在这里，我们描述了如何将边缘细胞簇的Airyscan图像转换为表面的3D模型，并检测凸曲率和凹曲率区域。我们使用光谱分解分析来比较不同基因型的表面纹理，以确定感兴趣的基因如何影响簇表面几何形状。该协议适用于边界细胞，并且可以推广到其他细胞类型。有关此协议的使用和执行的完整详细信息，PleaserefertoGabbertetal.1.
