Abstract：
Comparison of transcriptome for candidate gene discovery has become an important tool for biologists. While such studies lack the degree of resolution one gets from well-designed forward or reverse genetic studies, nevertheless, this has been a method of choice for giving coarse insight into the underlying biological processes or mechanisms. This was further accelerated with the availability of sequencing technologies. While many pipelines are available for RNA-seq data analysis, the protocol discussed here will guide the first-time users for conducting routine RNA-seq analysis using whole genome sequence as reference.
摘要：
比较转录组以发现候选基因已成为生物学家的重要工具。虽然这样的研究缺乏从精心设计的正向或反向遗传研究中获得的分辨率，然而，这是对潜在的生物过程或机制进行粗略了解的首选方法。这随着测序技术的可用性而进一步加速。虽然许多管道可用于RNA-seq数据分析，本文讨论的方案将指导首次使用者使用全基因组序列作为参考进行常规RNA-seq分析.
