Abstract：
Biological antibodies targeting key cytokines such as IL-17 and IL-23 have revolutionized psoriasis outcome. However, the recurrence remains an urgent challenge to be addressed. Currently, most of the descriptions of skin T-cell characteristics in psoriasis are derived from lesional and non-lesional skin, and their characteristics in resolved lesions (clinically healed lesions) remain vague. In order to further elucidate the cellular mechanism of recurrence, we performed single-cell sequencing and multiplexed immunohistochemical staining of T-cell subsets in autologous resolved lesion (RL), on-site recurrent psoriatic lesion (PL), and adjacent normal-appearing skin (NS) of psoriasis. By comparing with PL and NS tissues, we identified three potential cellular candidates for recurrence in clinically healed lesions: IL-17A/F double producing T cells, unstable Tregs and quiescent TRMs. Our results provide research clues for elucidating the immunological recurrence mechanism of psoriasis, and further work is needed to deepen our findings.
摘要：
靶向关键细胞因子如IL-17和IL-23的生物抗体已经彻底改变了银屑病的结果。然而,复发仍然是一个迫切需要解决的挑战。目前,牛皮癣中皮肤T细胞特征的大多数描述都来自皮损和非皮损，它们在已解决的病变（临床愈合的病变）中的特征仍然模糊。为了进一步阐明复发的细胞机制，我们对自体分辨病变（RL）中的T细胞亚群进行了单细胞测序和多重免疫组织化学染色，原位复发性银屑病病变（PL），和邻近的银屑病正常皮肤（NS）。通过与PL和NS组织的比较，我们确定了在临床愈合的病变中复发的三种潜在细胞候选物：IL-17A/F双产生T细胞，我们的研究结果为阐明银屑病的免疫复发机制提供了研究线索,需要进一步的工作来加深我们的发现。
