Abstract：
Three-dimensional (3D) cell culture techniques, which are superior to 2D methods in viability and functionality, are being used to develop innovative cancer vaccines. Tumor spheroids, which are structurally and functionally similar to actual tumors, can be developed using 3D cell culture. These spheroid vaccines have shown superior antitumor immune responses to 2D cell-based vaccines. Dendritic cell vaccines can also be produced more efficiently using 3D cell culture. Personalized cancer vaccines are being developed using 3D cell culture, providing substantial benefits over 2D methods. The more natural conditions of 3D cell culture might promote the expression of tumor antigens not expressed in 2D culture, potentially allowing for more targeted vaccines by co-culturing tumor cells with other cell types. Advanced cancer vaccines using 3D cell cultures are expected soon.
摘要：
三维（3D）细胞培养技术，在可行性和功能上优于2D方法，正在被用于开发创新的癌症疫苗。肿瘤球体,它们在结构上和功能上与实际的肿瘤相似，可以使用3D细胞培养开发。这些球体疫苗已经显示出优于基于2D细胞的疫苗的抗肿瘤免疫应答。还可以使用3D细胞培养更有效地生产树突状细胞疫苗。正在使用3D细胞培养开发个性化的癌症疫苗，与2D方法相比，提供了实质性的好处。更自然的3D细胞培养条件可能会促进2D培养中不表达的肿瘤抗原的表达，通过将肿瘤细胞与其他细胞类型共培养，可能允许更有针对性的疫苗。使用3D细胞培养的先进的癌症疫苗预计很快。
