Abstract：
Artemisia capillaris Thunb. (A. capillaris) is a well-known traditional Chinese herbal medicine with a wide range of pharmacological effects, such as soothing the liver and gallbladder, heat clearance, and detoxifying. Hence, its extract is commonly added to various traditional Chinese medicine formulas. Traditional Chinese medicine injection (TCMI) is a mature pharmaceutical dosage form developed using TCM theory combined with modern science and technology. Notably, allergic reactions, especially pseudo‑allergic reactions (PARs), greatly limited the use of these injections. Therefore, screening pseudo‑allergic components in A. capillaris extract is clinically significant. In the present study, we proposed a two-dimensional screening and identification system based on mas-related G protein-coupled receptor X2-HALO-tag/cell membrane chromatography (MrgX2-HALO-tag/CMC) high performance liquid chromatography mass spectrometry (HPLC-MS); seven potential active components were screened from 75 % ethanol extract of A. capillaris: NCA, CA, CCA, 1,3-diCQA, ICA-B, ICA-A, and ICA-C. The receptor-ligand interactions between these seven compounds and MrgX2 protein were analyzed using frontal analysis and molecular docking technology. Furthermore, a mast cell degranulation-related assay was used to assess the pseudo‑allergic activity of these compounds. The screened compounds can serve as ligands of MrgX2, and this study provides a research basis for pseudo‑allergic reactions caused by TCMIs containing A. capillaris.
摘要：
毛细血管蒿。(A.capillaris)是一种著名的传统中草药，具有广泛的药理作用，比如舒缓肝脏和胆囊，热间隙,和解毒。因此，它的提取物通常被添加到各种中药配方中。中药注射剂是运用中医理论结合现代科技发展起来的成熟药物剂型。值得注意的是,过敏反应,尤其是假性过敏反应（PAR），大大限制了这些注射剂的使用。因此,在毛细血管提取物中筛选假性过敏成分具有临床意义。在本研究中,我们提出了一种基于MAS相关G蛋白偶联受体X2-HALO-tag/细胞膜色谱（MrgX2-HALO-tag/CMC）高效液相色谱-质谱（HPLC-MS）的二维筛选和鉴定系统；从75％的辣椒A乙醇提取物中筛选出7个潜在的活性成分：NCA，CA,CCA,1,3-diCQA,ICA-B,ICA-A,和ICA-C使用正面分析和分子对接技术分析了这七个化合物与MrgX2蛋白之间的受体-配体相互作用。此外，肥大细胞脱粒相关测定法用于评估这些化合物的假性过敏活性。所筛选的化合物可作为MrgX2的配体，本研究为含TCMIs的TCMIs引起的假性过敏反应提供了研究基础。
