Abstract：
Legume plants establish an endosymbiosis with nitrogen-fixing rhizobia bacteria, which are taken up from the environment anew by each host generation. This requires a dedicated genetic program on the host side to control microbe invasion, involving coordinated reprogramming of host cells to create infection structures that facilitate inward movement of the symbiont. Infection initiates in the epidermis, with different legumes utilizing distinct strategies for crossing this cell layer, either between cells (intercellular infection) or transcellularly (infection thread infection). Recent discoveries on the plant side using fluorescent-based imaging approaches have illuminated the spatiotemporal dynamics of infection, underscoring the importance of investigating this process at the dynamic single-cell level. Extending fluorescence-based live-dynamic approaches to the bacterial partner opens the exciting prospect of learning how individual rhizobia reprogram from rhizospheric to a host-confined state during early root infection.
摘要：
豆科植物与固氮根瘤菌建立内共生关系，它们被每一代宿主重新从环境中吸收。这就需要宿主方面有专门的基因程序来控制微生物的入侵,涉及宿主细胞的协调重编程，以创建促进共生体向内运动的感染结构。感染始于表皮，不同的豆科植物利用不同的策略来穿越这个细胞层，细胞之间（细胞间感染）或跨细胞（感染线感染）。使用基于荧光的成像方法在植物方面的最新发现已经阐明了感染的时空动力学，强调在动态单细胞水平上研究这一过程的重要性。将基于荧光的实时动态方法扩展到细菌伴侣，为学习个体根瘤菌如何在早期根部感染期间从根际重新编程到宿主封闭状态开辟了令人兴奋的前景。
