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Abstract：
Astaxanthin is a red xanthophyll with high economic and industrial value in the pharmaceutical, nutraceutical, cosmetic and food industries. In recent years, the biotechnological production of astaxanthin has attracted much attention as a sustainable alternative to the predominating petrochemical-dependent chemical synthesis. In this regard, Xanthophyllomyces dendrorhous is regarded as a promising microorganism for industrial production of astaxanthin. Unfortunately, biotechnological production of the carotenoid is currently expensive. The present study investigated soy molasses (SM) and residual brewers\' yeast as cheap fermentation feedstocks for the cultivation of X. dendrorhous and astaxanthin production. Yeast extract was obtained from residual brewers\' yeast using various techniques and then combined with SM to formulate a two-component growth medium which was subsequently used to cultivate X. dendrorhous. Generally, the yeast extract produced from residual brewers\' yeast supported X. dendrorhous growth and astaxanthin production at levels comparable to those seen with commercial yeast extract. Overall, cultivating X. dendrorhous in an SM-based medium containing 5% SM and 0.2% yeast extract obtained from residual brewers\' yeast resulted in significantly higher (> 20% more) biomass accumulation compared to the control media (YPD). A similar slightly higher astaxanthin output (up to 14% more) was recorded in the SM-based medium compared to YPD. The formulated cultivation medium in this study provides an opportunity to reduce the production cost of astaxanthin from X. dendrorhous while simultaneously reducing the environmental impact related to the disposal of the industrial waste used as feedstock. KEY POINTS: • Cheap culture media were formulated from soy molasses and brewers\' spent yeast • The formulated medium resulted in at least 20% more biomass than the control • Up to 14% more astaxanthin was produced in molasses-based medium.
摘要：
虾青素是一种具有较高经济和工业价值的红色叶黄素,营养食品，化妆品和食品工业。近年来,虾青素的生物技术生产作为主要的石化依赖化学合成的可持续替代方法引起了广泛关注。在这方面,树状叶黄素菌被认为是工业生产虾青素的有前途的微生物。不幸的是,类胡萝卜素的生物技术生产目前是昂贵的。本研究调查了大豆糖蜜（SM）和残留的酿酒酵母作为廉价的发酵原料，用于培养X。树枝状和虾青素的生产。使用各种技术从残留的酿酒酵母中获得酵母提取物，然后与SM组合以配制双组分生长培养基，随后用于培养X。树枝状。一般来说,从剩余的酿酒酵母中产生的酵母提取物支持X.树突状生长和虾青素的产生，其水平与商业酵母提取物相当。总的来说,与对照培养基（YPD）相比，在含有5％SM和0.2％酵母提取物的SM基培养基中培养X。与YPD相比,在基于SM的培养基中记录到类似的稍高的虾青素输出(高达14%以上)。本研究中的配方培养基提供了一个机会，可以降低X树状虾青素的生产成本，同时减少与处理用作原料的工业废物有关的环境影响。关键点:•廉价培养基由大豆糖蜜和酿酒师配制。废酵母•配制的培养基导致比对照多至少20%的生物量•在基于糖蜜的培养基中产生多高达14%的虾青素。
