Abstract：
OBJECTIVE: Trimethyltin (TMT) chloride is an organotin compound used in industry. It has been linked to generating reactive oxygen species (ROS), inflammatory processes, and neuronal death. Carvacrol is a monoterpene phenol found in the Lamiaceae plant family, modulating inflammatory conditions and necroptosis in neural tissue. This study aimed to investigate the neuroprotective effects of carvacrol in a rat model of hippocampal neuronal injury induced by TMT.
METHODS: In this experimental study, sixty male Wistar rats were randomly divided into five groups (n=12): group 1 receiving saline, group 2 received dimethyl sulfoxide (DMSO) as a vehicle for 21 days, group 3 receiving a single dose of TMT (8 mg/kg) and groups 4 and 5 receiving carvacrol 40 and 70 mg/kg daily for 21 days after a single dose of TMT. All injections were intraperitoneal (I.P.). Caspase-3, Bax, Bcl-2, and Bdnf gene expression and the number of pyknotic neurons in the hippocampus were quantified. Spatial memory was assessed with a radial arm maze.
RESULTS: Statistical analysis of histological data revealed the carvacrol significantly attenuated cognitive dysfunction and the number of pyknotic neurons in the hippocampal CA1 region of rats treated with TMT. Based on real-time polymerase chain reaction (PCR), carvacrol modulated the expression of genes involved in apoptosis (Bax and Caspase-3) and upregulated anti-apoptotic (Bcl-2) and brain derived neurotrophic factor (Bdnf) genes in the hippocampal tissue.
CONCLUSIONS: These findings revealed neuroprotective effects of carvacrol which might be mediated by apoptotic and anti-apopetotic factors.
METHODS: In this experimental study, sixty male Wistar rats were randomly divided into five groups (n=12): group 1 receiving saline, group 2 received dimethyl sulfoxide (DMSO) as a vehicle for 21 days, group 3 receiving a single dose of TMT (8 mg/kg) and groups 4 and 5 receiving carvacrol 40 and 70 mg/kg daily for 21 days after a single dose of TMT. All injections were intraperitoneal (I.P.). Caspase-3, Bax, Bcl-2, and Bdnf gene expression and the number of pyknotic neurons in the hippocampus were quantified. Spatial memory was assessed with a radial arm maze.
RESULTS: Statistical analysis of histological data revealed the carvacrol significantly attenuated cognitive dysfunction and the number of pyknotic neurons in the hippocampal CA1 region of rats treated with TMT. Based on real-time polymerase chain reaction (PCR), carvacrol modulated the expression of genes involved in apoptosis (Bax and Caspase-3) and upregulated anti-apoptotic (Bcl-2) and brain derived neurotrophic factor (Bdnf) genes in the hippocampal tissue.
CONCLUSIONS: These findings revealed neuroprotective effects of carvacrol which might be mediated by apoptotic and anti-apopetotic factors.
摘要：
目的：氯化三甲基锡（TMT）是一种用于工业的有机锡化合物。它与产生活性氧（ROS）有关，炎症过程，和神经元死亡。香芹酚是在唇形科植物家族中发现的单萜酚，调节神经组织的炎症和坏死。本研究旨在探讨香芹酚对TMT诱导的大鼠海马神经元损伤的神经保护作用。
方法：在本实验研究中，将60只雄性Wistar大鼠随机分为5组(n=12):第1组接受生理盐水,第2组接受二甲亚砜（DMSO）作为载体21天，第3组接受单剂量TMT（8mg/kg），第4组和第5组每天接受香芹酚40和70mg/kg，持续21天。所有注射均为腹膜内注射(I.P.)。Caspase-3,Bax,对海马中Bcl-2和Bdnf基因的表达和细胞数量进行定量。用径向臂迷宫评估空间记忆。
结果：组织学数据的统计学分析显示，香芹酚可显着减轻TMT处理的大鼠的认知功能障碍和海马CA1区的固缩神经元数量。基于实时聚合酶链反应（PCR），香芹酚调节海马组织中与凋亡有关的基因（Bax和Caspase-3）的表达，并上调抗凋亡基因（Bcl-2）和脑源性神经营养因子（Bdnf）。
结论：这些发现揭示了香芹酚的神经保护作用，这可能是由凋亡和抗凋亡因子介导的。
方法：在本实验研究中，将60只雄性Wistar大鼠随机分为5组(n=12):第1组接受生理盐水,第2组接受二甲亚砜（DMSO）作为载体21天，第3组接受单剂量TMT（8mg/kg），第4组和第5组每天接受香芹酚40和70mg/kg，持续21天。所有注射均为腹膜内注射(I.P.)。Caspase-3,Bax,对海马中Bcl-2和Bdnf基因的表达和细胞数量进行定量。用径向臂迷宫评估空间记忆。
结果：组织学数据的统计学分析显示，香芹酚可显着减轻TMT处理的大鼠的认知功能障碍和海马CA1区的固缩神经元数量。基于实时聚合酶链反应（PCR），香芹酚调节海马组织中与凋亡有关的基因（Bax和Caspase-3）的表达，并上调抗凋亡基因（Bcl-2）和脑源性神经营养因子（Bdnf）。
结论：这些发现揭示了香芹酚的神经保护作用，这可能是由凋亡和抗凋亡因子介导的。
