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Abstract：
OBJECTIVE: To investigate the protective effect of the Chinese herbal formula of Jiedu Huayu decoction (, JHD) on oral mucosa of rats with oral submucosal fibrosis (OSF) and its potential mechanism of action.
METHODS: Sprague-Dawley male OSF model rats were constructed by injection of betaine and topical rubbing and were randomly grouped and administered by gavage for 4 weeks. Mouth opening and buccal mucosa scores interleukin levels and the expression of Axin and β-catenin proteins or genes were measured before and after drug administration.
RESULTS: After treatment with JHD the buccal mucosal lesions of rats were significantly reduced Axin protein and mRNA expression were significantly increased β-catenin protein and mRNA expression were significantly decreased interleukin-1β and interleukin-6 levels were decreased and interleukin-10 levels were increased.
CONCLUSIONS: The mechanism of action of JHD can effectively alleviate the pathological damage of buccal mucosa in OSF rats which may be related to the promotion of Axin expression and inhibition of β-catenin expression.
METHODS: Sprague-Dawley male OSF model rats were constructed by injection of betaine and topical rubbing and were randomly grouped and administered by gavage for 4 weeks. Mouth opening and buccal mucosa scores interleukin levels and the expression of Axin and β-catenin proteins or genes were measured before and after drug administration.
RESULTS: After treatment with JHD the buccal mucosal lesions of rats were significantly reduced Axin protein and mRNA expression were significantly increased β-catenin protein and mRNA expression were significantly decreased interleukin-1β and interleukin-6 levels were decreased and interleukin-10 levels were increased.
CONCLUSIONS: The mechanism of action of JHD can effectively alleviate the pathological damage of buccal mucosa in OSF rats which may be related to the promotion of Axin expression and inhibition of β-catenin expression.
摘要：
目的:探讨解毒化瘀汤(,JHD)对年夜鼠口腔黏膜下纤维化(OSF)的感化及其潜伏机制。
方法：采用甜菜碱注射和局部揉搓的方法构建Sprague-Dawley雄性OSF模型大鼠，随机分组，灌胃给药4周。在给药前后测量张口和颊粘膜评分的白细胞介素水平以及Axin和β-catenin蛋白或基因的表达。
结果：经JHD治疗后，大鼠颊黏膜病变的Axin蛋白和mRNA表达明显降低，β-catenin蛋白和mRNA表达明显升高，白细胞介素-1β和白细胞介素-6水平明显降低，白细胞介素-10水平升高。
结论：JHD可有效减轻OSF大鼠颊黏膜的病理损伤，其作用机制可能与促进Axin表达和抑制β-catenin表达有关。
方法：采用甜菜碱注射和局部揉搓的方法构建Sprague-Dawley雄性OSF模型大鼠，随机分组，灌胃给药4周。在给药前后测量张口和颊粘膜评分的白细胞介素水平以及Axin和β-catenin蛋白或基因的表达。
结果：经JHD治疗后，大鼠颊黏膜病变的Axin蛋白和mRNA表达明显降低，β-catenin蛋白和mRNA表达明显升高，白细胞介素-1β和白细胞介素-6水平明显降低，白细胞介素-10水平升高。
结论：JHD可有效减轻OSF大鼠颊黏膜的病理损伤，其作用机制可能与促进Axin表达和抑制β-catenin表达有关。
