Abstract：
The present study aimed to evaluate the single and combined effects of Si exogenous treatment and Bacillus subtilis subsp. subtilis M1 strain inoculation on rosemary tolerance to low phosphorus (P) availability. Hence, rosemary plants were fertilized with 250 µmol Ca3HPO4 (stressed plants) or 250 µmol KH2PO4 (control plants) under Si treatment and B. subtilis M1 strain inoculation. P starvation negatively affected rosemary growth and its P nutrition. However, exogenous Si supply or B. subtilis M1 strain inoculation significantly (P < 0.001) alleviated the deficiency-induced effects and significantly improved rhizogenesis, acid phosphatase activity, P uptake, and eventually dry weight of shoot and root. Moreover, Si-treatment and/or B. subtilis M1 strain inoculation significantly (P < 0.001) reduced the oxidative damage, in terms of malondialdehyde and hydrogen peroxide accumulation. This was found positively correlated with the higher superoxide dismutase activity, and the elevated non-enzymatic antioxidant molecules accumulation, including total polyphenols in Si-treated and inoculated P-deficient plants. Taken together, Si supplementation and/or B. subtilis M1 strain inoculation could be a good strategy to sustain rosemary plant growth under P starvation conditions.
摘要：
本研究旨在评估Si外源处理和枯草芽孢杆菌亚种的单一和联合作用。枯草杆菌M1菌株接种迷迭香对低磷利用率的耐受性。因此，在Si处理和枯草芽孢杆菌M1菌株接种下，用250µmolCa3HPO4（胁迫植物）或250µmolKH2PO4（对照植物）对迷迭香植物施肥。P饥饿对迷迭香的生长及其P营养产生负面影响。然而,外源Si供应或枯草芽孢杆菌M1菌株接种显着（p<0.001）减轻了缺陷诱导的影响，并显着改善了发根，酸性磷酸酶活性，磷的吸收和芽和根的最终干重。此外，Si处理和/或枯草芽孢杆菌M1菌株接种显着（p<0.001）降低了氧化损伤，在丙二醛和过氧化氢积累方面。发现这与较高的超氧化物歧化酶活性呈正相关，和升高的非酶抗氧化剂分子积累，包括Si处理和接种的P缺乏植物中的总多酚。一起来看,Si补充和/或枯草芽孢杆菌M1菌株接种可能是在P饥饿条件下维持迷迭香植物生长的良好策略。
