PDF(Pubmed)
Abstract：
Over the years, research regarding the Zika virus has been steadily increasing. Early immunization for ZIKV is a priority for preventing complications such as microencephaly and Guillain-Barré syndrome (GBS). Unlike traditional vaccination approaches, oral dissolving films (ODFs) or mucoadhesive film technology is an emerging, exciting concept that can be used in the field of pharmaceuticals for vaccine design and formulation development. This attractive and novel method can help patients who suffer from dysphagia as a complication of a disease or syndrome. In this study, we investigated a microparticulate Zika vaccine administered via the buccal route with the help of thin films or oral dissolving films (ODFs) with a prime dose and two booster doses two weeks apart. In vitro, the ODFs displayed excellent physiochemical properties, indicating that the films were good carriers for vaccine microparticles and biocompatible with the buccal mucosa. In vivo results revealed robust humoral (IgG, subtypes IgG1 and IgG2a) and T-cell responses (CD4+/CD8+) for ZIKV-specific immunity. Both the Zika MP vaccine and the adjuvanted Zika MP vaccine affected memory (CD45R/CD27) and intracellular cytokine (TNF-α and IL-6) expression. In this study, ZIKV vaccination via the buccal route with the aid of ODFs demonstrated great promise for the development of pain-free vaccines for infectious diseases.
摘要：
多年来,关于寨卡病毒的研究一直在稳步增长。ZIKV的早期免疫是预防小脑症和格林-巴利综合征（GBS）等并发症的优先事项。与传统的疫苗接种方法不同，口腔溶解膜(ODF)或粘膜粘附膜技术是一种新兴的，令人兴奋的概念，可用于药物领域的疫苗设计和制剂开发。这种有吸引力的新颖方法可以帮助患有吞咽困难作为疾病或综合征的并发症的患者。在这项研究中,我们研究了一种微粒寨卡疫苗,该疫苗通过口腔途径给药,并借助薄膜或口服溶解薄膜(ODF),一次主要剂量和两次加强剂量,间隔两周.体外，ODF表现出优异的理化性质，表明该薄膜是疫苗微粒的良好载体，并且与颊粘膜具有生物相容性。体内结果显示强健的体液(IgG，亚型IgG1和IgG2a）和ZIKV特异性免疫的T细胞反应（CD4/CD8）。寨卡MP疫苗和佐剂化的寨卡MP疫苗都影响记忆(CD45R/CD27)和胞内细胞因子(TNF-α和IL-6)表达。在这项研究中,在ODF的帮助下通过口腔途径进行的ZIKV疫苗接种显示出开发用于传染病的无痛疫苗的巨大前景。
