PDF(Pubmed)
Abstract：
Triple-negative breast cancer (TNBC) is an aggressive subtype characterized by the absence of estrogen receptor, progesterone receptor, and human epidermal growth factor receptor type 2 expression. It is known for its high malignancy, invasiveness, and propensity for metastasis, resulting in a poor prognosis due to the absence of beneficial therapeutic targets. Natural products derived from mushrooms have gained significant attention in neoplastic therapy due to their potential medicinal properties. The therapeutic potential of Ganoderma lucidum in breast cancer has been highlighted by our group, suggesting its use as an adjuvant treatment. The present study aims to assess the potential antineoplastic capacity of two Caribbean native Ganoderma species found in Puerto Rico, Ganoderma multiplicatum (G. multiplicatum) and Ganoderma martinicense (G. martinicense). Antiproliferative studies were conducted via cell viability assays after cultivation, harvesting, and fractionation of both species. The obtained results indicate that most of the fractions show some cytotoxicity against all cell lines, but 33% of the fractions (F1, F2, F7, F12) display selectivity towards cancer cell models. We demonstrate for the first time that native Ganoderma species can generate metabolites with anti-TNBC properties. Future avenues will focus on structure elucidation of the most active fractions of these Ganoderma extracts.
摘要：
三阴性乳腺癌（TNBC）是一种侵袭性亚型，其特征是缺乏雌激素受体，孕激素受体,和人表皮生长因子受体2型表达。它以其高度恶性而闻名，侵入性,和转移倾向，由于缺乏有益的治疗靶标，导致预后不良。源自蘑菇的天然产物由于其潜在的药用特性而在肿瘤治疗中获得了极大的关注。灵芝在乳腺癌中的治疗潜力已被我们小组强调，建议将其用作辅助治疗。本研究旨在评估在波多黎各发现的两种加勒比海本地灵芝物种的潜在抗肿瘤能力，多毛灵芝(G.Multiicatum)和马丁尼克灵芝(G.马丁尼克）。抗增殖研究通过培养后的细胞活力测定进行，收获,和两个物种的分馏。获得的结果表明，大多数级分对所有细胞系都显示出一定的细胞毒性，但33%的级分(F1、F2、F7、F12)显示对癌细胞模型的选择性。我们首次证明了天然灵芝物种可以产生具有抗TNBC特性的代谢物。未来的途径将集中在这些灵芝提取物中最活跃的部分的结构阐明上。
