PDF(Pubmed)
Abstract：
In this study, UHPLC-HRMS analysis of the defatted methanol extract obtained from Inula salicina L. led to the identification of 58 compounds-hydroxycinnamic and hydroxybenzoic acids and their glycosides, acylquinic and caffeoylhexaric acids, and flavonoids and their glycosides. In addition, a new natural compound, N-(8-methylnepetin)-3-hydroxypiperidin-2-one was isolated and its structure was elucidated by NMR spectroscopy. The presence of a flavoalkaloid in genus Inula is described now for the first time. Chlorogenic acid was the main compound followed by 3,5-, 1,5- and 4,5-dicaffeoylquinic acids. The methanol extract was studied for its antioxidant potential by DPPH, ABTS, and FRAP assays and sun protective properties. In addition, a study was conducted to assess the effectiveness of the tested extract in inhibiting biofilm formation by Gram-positive and Gram-negative strains. Results from crystal violet tests revealed a notable decrease in biofilm mass due to the extract. The anti-biofilm efficacy was confirmed through the observation of the biofilm viability by live/dead staining. The obtained results showed that this plant extract could be used in the development of cosmetic products with antibacterial and sun protection properties.
摘要：
在这项研究中,从InulasalicinaL.获得的脱脂甲醇提取物的UHPLC-HRMS分析导致鉴定了58种化合物-羟基肉桂酸和羟基苯甲酸及其糖苷，酰基奎尼酸和咖啡酰基己二酸，和类黄酮及其糖苷。此外,一种新的天然化合物,分离了N-（8-甲基萘佩汀）-3-羟基哌啶-2-酮，并通过NMR光谱阐明了其结构。现在首次描述了Inula属中黄体生物碱的存在。绿原酸是主要化合物，其次是3,5-，1,5-和4,5-二咖啡酰基奎尼酸。通过DPPH研究了甲醇提取物的抗氧化潜力，ABTS,和FRAP测定和防晒性能。此外,进行了一项研究以评估受试提取物抑制革兰氏阳性和革兰氏阴性菌株生物膜形成的有效性。结晶紫试验的结果显示,由于提取物,生物膜质量显著降低。通过活/死染色观察生物膜活力来确认抗生物膜功效。获得的结果表明，该植物提取物可用于开发具有抗菌和防晒性能的化妆品。
