PDF(Pubmed)
Abstract：
Hydraulic fracturing, or fracking, requires large amounts of water to extract fossil fuel from rock formations. As a result of hydraulic fracturing, the briny wastewater, often termed back-produced fracturing or fracking water (FW), is pumped into holding ponds. One of the biggest challenges with produced water management is controlling microbial activity that could reduce the pond water\'s reusable layer and pose a significant environmental hazard. This study focuses on the characterization of back-produced water that has been hydraulically fractured using chemical and biological analysis and the development of a high-throughput screening method to evaluate and predict the antimicrobial effect of four naturally and commercially available acidic inhibitors (edetic acid, boric acid, tannic acid, and lactic acid) on the growth of the FW microbiome. Liquid cultures and biofilms of two laboratory model strains, the vegetative Escherichia coli MG1655, and the spore-forming Bacillus atrophaeus (also known as Bacillus globigii, BG) bacteria, were used as reference microorganisms. Planktonic bacteria in FW were more sensitive to antimicrobials than sessile bacteria in biofilms. Spore-forming BG bacteria exhibited more sensitivity to acidic inhibitors than the vegetative E. coli cells. Organic acids were the most effective bacterial growth inhibitors in liquid culture and biofilm.
摘要：
水力压裂,或者水力压裂，需要大量的水来从岩层中提取化石燃料。由于水力压裂，含盐的废水，通常被称为返产压裂或压裂水(FW)，被泵入蓄水池。生产水管理的最大挑战之一是控制微生物活动，这可能会减少池塘水的可重复使用层，并对环境造成重大危害。本研究的重点是使用化学和生物分析对水力压裂的回采水进行表征，并开发了一种高通量筛选方法，以评估和预测四种天然和市售酸性抑制剂（乙二胺四乙酸，硼酸,鞣酸,和乳酸）对FW微生物组的生长。两种实验室模型菌株的液体培养物和生物膜，营养性大肠杆菌MG1655，和孢子形成的萎缩芽孢杆菌（也称为球形芽孢杆菌，BG)细菌，用作参考微生物。FW中的浮游细菌比生物膜中的固着细菌对抗菌剂更敏感。与营养性大肠杆菌细胞相比,形成孢子的BG细菌对酸性抑制剂表现出更高的敏感性。有机酸是液体培养和生物膜中最有效的细菌生长抑制剂。
