PDF(Pubmed)
Abstract：
This study aimed to determine shifts in microbial populations regarding richness and diversity from the daily use of a popular over-the-counter nasal spray. In addition, the finding of nasal commensal bacterial species that overlap with the oral microbiome may prove to be potential probiotics for the \"gateway microbiomes\". Nasal swab samples were obtained before and after using the most popular over-the-counter (OTC) nasal spray in 10 participants aged 18-48. All participants were healthy volunteers with no significant medical histories. The participants were randomly assigned a number by randomizing software and consisted of five men and five women. The sampling consisted of placing a nasal swab atraumatically into the nasal cavity. The samples were preserved and sent to Northwestern University Sequencing Center for whole-genome deep sequencing. After 21 days of OTC nasal spray use twice daily, the participants returned for further nasal microbiome sampling. The microbial analysis included all bacteria, archaea, viruses, molds, and yeasts via deep sequencing for species analysis. The Northwestern University Sequencing Center utilized artificial intelligence analysis to determine shifts in species and strains following nasal spray use that resulted in changes in diversity and richness.
摘要：
这项研究旨在从日常使用流行的非处方鼻喷雾剂中确定微生物种群在丰富度和多样性方面的变化。此外,与口腔微生物组重叠的鼻腔共生细菌的发现可能被证明是“网关微生物组”的潜在益生菌。在10名年龄在18-48岁的参与者中使用最受欢迎的非处方（OTC）鼻喷雾剂之前和之后获得鼻拭子样本。所有参与者均为健康志愿者，无明显病史。参与者通过随机软件随机分配了一个数字，由五名男性和五名女性组成。取样包括将鼻拭子无创伤地放入鼻腔。将样品保存并送到西北大学测序中心进行全基因组深度测序。经过21天的OTC鼻腔喷雾剂每天两次，参与者返回进行进一步的鼻腔微生物组采样.微生物分析包括所有细菌，古细菌,病毒,模具，和酵母通过深度测序进行物种分析。西北大学测序中心利用人工智能分析来确定鼻腔喷雾使用后物种和菌株的变化，从而导致多样性和丰富度的变化。
