PDF(Pubmed)
Abstract：
In previous studies, it was demonstrated that Corynebacterium pseudodiphtheriticum 090104, isolated from the human nasopharynx, modulates respiratory immunity, improving protection against infections. Here, the antagonistic effect of the 090104 strain on respiratory pathogens, including Streptococcus pneumoniae, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii, was explored. In a series of in vitro studies, the capacity of C. pseudodiphtheriticum 090104, its bacterium-like particles, and its culture supernatants to coaggregate, inhibit the growth, and change the virulent phenotype of pathogenic bacteria was evaluated. The results showed that the 090104 strain was able to exert a bacteriostatic effect on K. pneumoniae and S. pneumoniae growth. In addition, C. pseudodiphtheriticum 090104 coaggregated, inhibited biofilm formation, and induced phenotypic changes in all the respiratory pathogens evaluated. In conclusion, this work demonstrated that, in addition to its beneficial effects exerted by host-microbe interactions, C. pseudodiphtheriticum 090104 can enhance protection against respiratory pathogens through its microbe-microbe interactions. The mechanisms involved in such interactions should be evaluated in future research.
摘要：
在以往的研究中,证明了从人鼻咽中分离出的假白喉棒状杆菌090104，调节呼吸免疫力，提高对感染的保护。这里,090104菌株对呼吸道病原体的拮抗作用，包括肺炎链球菌,金黄色葡萄球菌,肺炎克雷伯菌,铜绿假单胞菌,鲍曼不动杆菌,被探索了。在一系列体外研究中，假白喉梭菌090104，其类细菌颗粒的容量，和它的培养上清液共同聚集，抑制生长，并评价病原菌毒力表型的变化。结果表明,090104菌株能够对肺炎克雷伯菌和肺炎链球菌的生长发挥抑菌作用。此外,C.假白喉090104共聚集，抑制生物膜形成，并在所有评估的呼吸道病原体中诱导表型变化。总之,这项工作表明，除了其通过宿主-微生物相互作用发挥的有益作用外，拟白喉梭菌090104可以通过其微生物-微生物相互作用增强对呼吸道病原体的保护。这种相互作用所涉及的机制应在未来的研究中进行评估。
