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Abstract：
Although cases of Legionnaires\' disease are notifiable, data on the phenotypic and genotypic characterisation of clinical isolates are limited. This retrospective study aims to report the results of the characterisation of Legionella clinical isolates in Spain from 2012 to 2022. Monoclonal antibodies from the Dresden panel were used for phenotypic identification of Legionella pneumophila. Genotypic characterisation and sequence type assignment were performed using the Sequence-Based Typing scheme. Of the 1184 samples, 569 were identified as Legionella by culture. Of these, 561 were identified as L. pneumophila, of which 521 were serogroup 1. The most common subgroups were Philadelphia (n = 107) and Knoxville (n = 106). The SBT analysis revealed 130 different STs, with the most common genotypes being ST1 (n = 87), ST23 (n = 57), ST20 (n = 30), and ST42 (n = 29). Knoxville has the highest variability with 32 different STs. ST23 is mainly found in Allentown/France (n = 46) and ST42 in Benidorm (n = 18), whereas ST1 is widely distributed. The results demonstrate that clinical isolates show high genetic diversity, although only a few sequence types (STs) are responsible for most cases. However, outbreaks can also occur with rare genotypes. More data on LD and associated epidemiological studies are needed to establish the risk of an isolate causing outbreak in the future.
摘要：
虽然军团病病例是应报告的，关于临床分离株的表型和基因型表征的数据有限.这项回顾性研究旨在报告2012年至2022年西班牙军团菌临床分离株的表征结果。来自德累斯顿小组的单克隆抗体用于嗜肺军团菌的表型鉴定。使用基于序列的分型方案进行基因型表征和序列类型分配。在1184个样本中,569人通过培养鉴定为军团菌。其中,561人被鉴定为嗜肺乳杆菌，其中521个是血清组1。最常见的亚组是费城（n=107）和诺克斯维尔（n=106）。SBT分析揭示了130种不同的ST，最常见的基因型是ST1（n=87），ST23（n=57），ST20（n=30），和ST42(n=29)。诺克斯维尔在32种不同的ST中具有最高的变异性。ST23主要在法国艾伦敦（n=46）和ST42在贝尼多姆（n=18），而ST1分布广泛。结果表明，临床分离株具有较高的遗传多样性，尽管只有少数序列类型(ST)负责大多数情况。然而,罕见基因型也可能爆发。需要更多有关LD和相关流行病学研究的数据，以确定将来隔离引起爆发的风险。
