PDF(Pubmed)
Abstract：
Angomonas deanei belongs to Trypanosomatidae family, a family of parasites that only infect insects. It hosts a bacterial endosymbiont in a mutualistic relationship, constituting an excellent model for studying organelle origin and cellular evolution. A lipidomic approach, which allows for a comprehensive analysis of all lipids in a biological system (lipidome), is a useful tool for identifying and measuring different expression patterns of lipid classes. The present study applied GC-MS and NMR techniques, coupled with principal component analysis (PCA), in order to perform a comparative lipidomic study of wild and aposymbiotic A. deanei grown in the presence or absence of FBS. Unusual contents of branched-chain iso C17:0 and C19:0-cis-9,10 and-11,12 fatty acids were identified in A. deanei cultures, and it was interesting to note that their content slightly decreased at the log phase culture, indicating that in the latter growth stages the cell must promote the remodeling of lipid synthesis in order to maintain the fluidity of the membrane. The combination of analytical techniques used in this work allowed for the detection and characterization of lipids and relevant contributors in a variety of A. deanei growth conditions.
摘要：
安氏单胞菌属于锥虫科，只感染昆虫的寄生虫家族。它以互惠的关系拥有细菌内共生体，构成了研究细胞器起源和细胞进化的极好模型。脂质组学方法，它可以全面分析生物系统中的所有脂质（脂质），是用于识别和测量脂质类别的不同表达模式的有用工具。本研究应用GC-MS和NMR技术，结合主成分分析(PCA)，为了对在存在或不存在FBS的情况下生长的野生和非生动物A.deanei进行比较脂质组学研究。在A.deanei培养物中鉴定出支链异C17：0和C19：0-顺式-9,10和-11,12脂肪酸的含量异常，有趣的是，它们的含量在对数阶段文化中略有下降，表明在后期生长阶段，细胞必须促进脂质合成的重塑，以维持膜的流动性。这项工作中使用的分析技术的组合允许在各种A.deanei生长条件下检测和表征脂质和相关贡献者。
