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Abstract：
Chysobalanus icaco L. (C. icaco) is a plant that is native to tropical America and Africa. It is also found in the southeast region of Mexico, where it is used as food and to treat certain diseases. This study aimed to carry out a phytochemical analysis of an aqueous extract of C. icaco seed (AECS), including its total phenol content (TPC), total flavonoid content (TFC), and condensed tannins (CT). It also aimed to examine the antioxidant and metal-ion-reducing potential of the AECS in vitro, as well as its toxicity and anti-inflammatory effect in mice. Antioxidant and metal-ion-reducing potential was examined by inhibiting DPPH, ABTS, and FRAP. The acute toxicity test involved a single administration of different doses of the AECS (0.5, 1, and 2 g/kg body weight). Finally, a single administration at doses of 150, 300, and 600 mg/kg of the AECS was used in the carrageenan-induced model of subplantar acute edema. The results showed that the AECS contained 124.14 ± 0.32 mg GAE, 1.65 ± 0.02 mg EQ, and 0.910 ± 0.01 mg of catechin equivalents/g dried extract (mg EC/g de extract) for TPC, TFC and CT, respectively. In the antioxidant potential assays, the values of the median inhibition concentration (IC50) of the AECS were determined with DPPH (0.050 mg/mL), ABTS (0.074 mg/mL), and FRAP (0.49 mg/mL). Acute toxicity testing of the AECS revealed no lethality, with a median lethal dose (LD50) value of >2 g/kg by the intragastric route. Finally, for inhibition of acute edema, the AECS decreased inflammation by 55%, similar to indomethacin (59%, p > 0.05). These results demonstrated that C. icaco seed could be considered a source of bioactive molecules for therapeutic purposes due to its antioxidant potential and anti-inflammatory activity derived from TPC, with no lethal effect from a single intragastric administration in mice.
摘要：
ChysobalanusicacoL.（C.icaco）是一种原产于热带美洲和非洲的植物。它也在墨西哥的东南部地区发现，它被用作食物和治疗某些疾病。本研究旨在对icaco种子（AECS）的水提取物进行植物化学分析，包括其总酚含量(TPC)，总黄酮含量（TFC），和缩合单宁（CT）。它还旨在检查体外AECS的抗氧化剂和金属离子还原潜力，以及其对小鼠的毒性和抗炎作用。通过抑制DPPH检查抗氧化剂和金属离子还原电位，ABTS,和FRAP。急性毒性试验涉及单次施用不同剂量的AECS(0.5、1和2g/kg体重)。最后,在角叉菜胶诱导的足下急性水肿模型中,使用150,300和600mg/kg剂量的AECS单次给药.结果表明，AECS含有124.14±0.32mgGAE，1.65±0.02mgEQ，和0.910±0.01mg儿茶素当量/g干燥提取物（mgEC/gde提取物）用于TPC，TFC和CT,分别。在抗氧化潜力测定中，用DPPH（0.050mg/mL）测定AECS的中位抑制浓度（IC50）值，ABTS（0.074mg/mL），和FRAP(0.49mg/mL)。AECS的急性毒性测试显示没有致死性，通过胃内途径的中位致死剂量（LD50）值>2g/kg。最后,用于抑制急性水肿，AECS减少了55%的炎症，与吲哚美辛相似(59%，p>0.05)。这些结果表明，由于其抗氧化潜力和源自TPC的抗炎活性，icaco种子可被认为是用于治疗目的的生物活性分子的来源。对小鼠单次灌胃没有致死作用。
