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Abstract：
Background and Objectives: Parathyroid adenoma is a distinct cause of primary hyperparathyroidism, with the vast majority being sporadic ones. Proteomic analysis of parathyroid adenomas has proposed a large number of related proteins. The aim of this study is to evaluate the immunohistochemical staining of ANXA2, MED12, MAPK1 and VDR in parathyroid adenoma tissue. Materials and Methods: Fifty-one parathyroid adenomas were analyzed for ANXA2, MED12, MAPK1 and VDR expressions. Tissue was extracted from formalin-fixed paraffin-embedded parathyroid adenoma specimens; an immunohistochemical study was applied, and the percentage of allocation and intensity were evaluated. Results: ANXA2 stained positively in 60.8% of all cell types, while MED12 had positive staining in 66%. MAPK1 expression was found to be negative in total, although a specific pattern for oxyphil cells was observed, as they stained positive in 17.7%. Finally, VDR staining was positive at 22.8%, based on nuclear staining. Conclusions: These immunohistochemical results could be utilized as biomarkers for the diagnosis of sporadic parathyroid adenoma. It is of great importance that a distinct immunophenotype of nodule-forming cells in a positive adenoma could suggest a specific pattern of adenoma development, as in hereditary patterns.
摘要：
背景和目的:甲状旁腺腺瘤是原发性甲状旁腺功能亢进症的独特病因,绝大多数是零星的。甲状旁腺腺瘤的蛋白质组学分析提出了大量相关蛋白。本研究旨在评估甲状旁腺腺瘤组织中ANXA2，MED12，MAPK1和VDR的免疫组织化学染色。材料与方法：分析51例甲状旁腺腺瘤中ANXA2、MED12、MAPK1和VDR的表达。从福尔马林固定石蜡包埋的甲状旁腺腺瘤标本中提取组织；应用免疫组织化学研究，并对分配的百分比和强度进行了评估。结果：ANXA2在所有细胞类型的60.8％中呈阳性，而66％的MED12染色呈阳性。发现MAPK1表达总体为阴性，尽管观察到了嗜氧细胞的特定模式，因为它们在17.7%中染色为阳性。最后,VDR染色阳性22.8%,基于核染色。结论：这些免疫组化结果可作为散发性甲状旁腺腺瘤诊断的生物标志物。非常重要的是，阳性腺瘤中结节形成细胞的独特免疫表型可以提示腺瘤发展的特定模式。就像遗传模式一样。
