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Abstract：
In the field of biomedicine, efficiently and non-invasively isolating target cells has always been one of the core challenges. Optical fiber tweezers offer precise and non-invasive manipulation of cells within a medium and can be easily integrated with microfluidic systems. Therefore, this paper investigated the mechanism of cell manipulation using scattering force with optical fiber tweezers. We employed flat-ended single-mode fiber to drive and sort cells and derived the corresponding scattering force formula based on the T-matrix model. A single-mode optical tweezers system for cell sorting was developed, and an optofluidic experimental platform was constructed that effectively integrates the optical system with microfluidic chips. The chip, featuring an expanded cross-channel design, successfully achieved continuous separation of yeast cells (8~10 µm in diameter) and polystyrene microspheres (15~20 µm in diameter), with a sorting efficiency of up to 86% and maintaining viability in approximately 90% of the yeast cells. Compared to other sorting systems, this system does not require labeling and can achieve continuous sorting with cell viability at a lower cost of instrumentation.
摘要：
在生物医学领域,有效和非侵入性地分离靶细胞一直是核心挑战之一。光纤镊子提供对培养基中细胞的精确和非侵入性操作，并且可以很容易地与微流体系统集成。因此,本文研究了光纤镊子利用散射力操纵细胞的机理。我们使用平端单模光纤驱动和分选细胞，并基于T矩阵模型得出相应的散射力公式。开发了一种用于细胞分选的单模光学镊子系统，并构建了光流控实验平台，将光学系统与微流控芯片有效集成。芯片，具有扩展的跨通道设计，成功实现了酵母细胞(直径8~10μm)和聚苯乙烯微球(直径15~20μm)的连续分离,分选效率高达86%，并在大约90%的酵母细胞中保持活力。与其他分拣系统相比，该系统不需要标记，并且可以以较低的仪器成本实现具有细胞活力的连续分选。
