PDF(Pubmed)
Abstract：
In August 2023, we identified a case of dengue fever in Yantai City, which was imported from Xishuangbanna, China. To investigate its evolutionary history and population dynamics, we utilized the metatranscriptomic method to obtain the virus\' whole genome sequence. Together with 367 selected dengue virus whole genome sequences from the NCBI database, we constructed a time-scaled Maximum Clade Credibility (MCC) tree. We found that our sequence exhibited a high homology with a sequence of DENV1 (OR418422.1) uploaded by the Guangzhou Center for Disease Control and Prevention in 2023, with an estimated divergence time around 2019 (95% HPD: 2017-2023), coinciding with the emergence of SARS-CoV-2. The DENV strain obtained in this study belongs to genotype I of DENV1. Its ancestors experienced a global epidemic around 2005 (95% HPD: 2002-2010), and its progeny strains have spread extensively in Southeast Asia and China since around 2007 (95% HPD: 2006-2011). The Bayesian skyline plot indicates that the current population of DENV1 has not been affected by SARS-CoV-2 and is expected to maintain stable transmission. Hence, it is imperative to track and monitor its epidemiological trends and genomic variations to prevent potential large-scale outbreaks in the post-SARS-CoV-2 era.
摘要：
2023年8月，我们在烟台市发现了一例登革热病例，是从西双版纳进口的，中国。为了研究其进化历史和种群动态，我们利用转移转录组学方法获得了病毒的全基因组序列。连同来自NCBI数据库的367种选定的登革热病毒全基因组序列，我们构建了一个时间尺度的最大分化可信度(MCC)树。我们发现我们的序列与2023年广州市疾病预防控制中心上传的DENV1（OR418422.1）序列具有高度同源性，估计发散时间在2019年左右（95％HPD：2017-2023），与SARS-CoV-2的出现相吻合。本研究中获得的DENV菌株属于DENV1的基因型I。它的祖先在2005年左右经历了一次全球流行病（95％的HPD：2002-2010），自2007年左右(95%HPD:2006-2011)以来，其后代菌株已在东南亚和中国广泛传播。贝叶斯天际线图表明，DENV1的当前种群并未受到SARS-CoV-2的影响，并有望保持稳定的传播。因此，必须跟踪和监测其流行病学趋势和基因组变异，以防止SARS-CoV-2后时代潜在的大规模爆发。
