PDF(Pubmed)
Abstract：
In recent years, due to the large Romanian community present in Italy, the retail of foods coming from Eastern Europe has increased. The most common type of violation detected in these foods consists of incorrect labeling and species-replacement frauds. In this paper, the compliance of labels of 43 ethnic processed food coming from Eastern Europe and commercialized in Italy was evaluated by means of PCR and Sanger sequencing. Our data revealed 33% of non-compliant labels in samples containing swine, ruminants, and avian ingredients. These results demonstrate that PCR can be easily used for the identification of species in highly processed products, proving to be a rapid, effective, and economic method. On the other hand, samples reporting fish as ingredients highlighted the ineffectiveness of the applied sequencing protocol, due to the low informative property of targeted fragments or to the lack of consensus sequences in the case of uncommon species.
摘要：
近年来,由于意大利有大量的罗马尼亚社区，来自东欧的食品零售额有所增加。在这些食品中发现的最常见的违规行为包括错误的标签和物种替代欺诈。在本文中,通过PCR和Sanger测序评估了来自东欧并在意大利商业化的43种民族加工食品的标签合规性。我们的数据显示，含有猪的样本中有33%的不合规标签，反刍动物,和鸟类成分。这些结果表明，PCR可以很容易地用于鉴定高度加工产品中的物种，证明是一个快速的，有效,和经济方法。另一方面,报告鱼作为成分的样本强调了应用测序方案的无效性，由于目标片段的信息特性低或在不常见物种的情况下缺乏共有序列。
